Schneweis K E, Kleim J P, Bailly E, Niese D, Wagner N, Brackmann H H
Institute of Medical Microbiology and Immunology, University of Bonn, Federal Republic of Germany.
Med Microbiol Immunol. 1990;179(4):193-203. doi: 10.1007/BF00195250.
To determine whether the biological variability of HIV-1 has any clinical significance, the highly variable cytopathogenicity of 153 HIV-1 strains, isolated from 119 hemophiliacs, was related to the number of CD4+ lymphocytes present in the patient's blood at the time of virus isolation. It was shown that the cytopathogenicity of the HIV-1 isolates was inversely correlated with the number of CD4+ lymphocytes. The highest CD4+ cell number were observed in 34 latently infected patients characterized by HIV seropositivity, failure of virus isolation, and detection of viral DNA by the polymerase chain reaction. Cytopathogenicity of the HIV-1 isolates was a reliable prognostic marker and correlated well with other less-sensitive prognostic parameters, including the detection of infectious virus and p24 antigen in the plasma, and the decline of p24 antibody in the serum. The results suggest that the viral isolates - if not subjected to extensive passage - represent in vivo variants selected from a heterogeneous viral population according to the particular immunological conditions of the host.
为了确定HIV-1的生物学变异性是否具有临床意义，从119名血友病患者中分离出153株HIV-1毒株，研究其高度可变的细胞致病性与病毒分离时患者血液中CD4+淋巴细胞数量的关系。结果表明，HIV-1分离株的细胞致病性与CD4+淋巴细胞数量呈负相关。在34例以HIV血清阳性、病毒分离失败和通过聚合酶链反应检测到病毒DNA为特征的潜伏感染患者中，观察到最高的CD4+细胞数。HIV-1分离株的细胞致病性是一个可靠的预后标志物，与其他敏感性较低的预后参数密切相关，包括血浆中感染性病毒和p24抗原的检测，以及血清中p24抗体的下降。结果表明，如果不进行广泛传代，病毒分离株代表根据宿主特定免疫条件从异质性病毒群体中选择的体内变体。
