酿酒酵母中Spo11-寡核苷酸复合物的末端标记与分析。
End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae.
作者信息
Neale Matthew J, Keeney Scott
机构信息
MRC Genome Damage and Stability Centre, University of Sussex, Falmer, UK.
出版信息
Methods Mol Biol. 2009;557:183-95. doi: 10.1007/978-1-59745-527-5_12.
Abstract
During meiosis Spo11 catalyzes the formation of DNA double-strand breaks, becoming covalently attached to the 5' ends on both sides of the break during this process. Spo11 is removed from the DSB by single-stranded endonucleolytic cleavage flanking the DSB, liberating a short-lived species consisting of Spo11 protein covalently linked to a short oligonucleotide. The method presented here details how to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells.
摘要
在减数分裂过程中，Spo11催化DNA双链断裂的形成，并在此过程中与断裂两侧的5'端共价连接。通过双链断裂侧翼的单链内切核酸酶切割，Spo11从双链断裂处被移除，释放出一种由Spo11蛋白与短寡核苷酸共价连接组成的短暂存在的物质。本文介绍的方法详细说明了如何在减数分裂酵母细胞提取物中检测这些Spo11-寡核苷酸复合物。
相似文献
1
End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae.酿酒酵母中Spo11-寡核苷酸复合物的末端标记与分析。
Methods Mol Biol. 2009;557:183-95. doi: 10.1007/978-1-59745-527-5_12.
2
Detection of SPO11-oligonucleotide complexes from mouse testes.从小鼠睾丸中检测SPO11 - 寡核苷酸复合物。
Methods Mol Biol. 2009;557:197-207. doi: 10.1007/978-1-59745-527-5_13.
3
Endonucleolytic processing of covalent protein-linked DNA double-strand breaks.共价蛋白连接的DNA双链断裂的核酸内切加工
Nature. 2005 Aug 18;436(7053):1053-7. doi: 10.1038/nature03872.
4
CC-seq: Nucleotide-Resolution Mapping of Spo11 DNA Double-Strand Breaks in S. cerevisiae Cells.CC-Seq：酿酒酵母细胞中 Spo11 双链断裂的核苷酸分辨率作图
Methods Mol Biol. 2024;2818:3-22. doi: 10.1007/978-1-0716-3906-1_1.
5
Locally, meiotic double-strand breaks targeted by Gal4BD-Spo11 occur at discrete sites with a sequence preference.在局部区域，由Gal4BD-Spo11靶向的减数分裂双链断裂发生在具有序列偏好性的离散位点。
Mol Cell Biol. 2009 Jul;29(13):3500-16. doi: 10.1128/MCB.00088-09. Epub 2009 Apr 20.
6
Targeted induction of meiotic double-strand breaks reveals chromosomal domain-dependent regulation of Spo11 and interactions among potential sites of meiotic recombination.减数分裂双链断裂的靶向诱导揭示了Spo11的染色体结构域依赖性调控以及减数分裂重组潜在位点之间的相互作用。
Nucleic Acids Res. 2008 Feb;36(3):984-97. doi: 10.1093/nar/gkm1082. Epub 2007 Dec 20.
7
Modulating and targeting meiotic double-strand breaks in Saccharomyces cerevisiae.调控并靶向酿酒酵母中的减数分裂双链断裂
Methods Mol Biol. 2009;557:27-33. doi: 10.1007/978-1-59745-527-5_3.
8
Structural and functional characterization of the Spo11 core complex.Spo11 核心复合物的结构与功能特征分析。
Nat Struct Mol Biol. 2021 Jan;28(1):92-102. doi: 10.1038/s41594-020-00534-w. Epub 2021 Jan 4.
9
Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases.减数分裂 DNA 双链断裂的处理需要细胞周期蛋白依赖性激酶和多种核酸酶。
J Biol Chem. 2010 Apr 9;285(15):11628-37. doi: 10.1074/jbc.M110.104083. Epub 2010 Feb 11.
10
Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomes.Rec8引导酵母减数分裂染色体上的典型Spo11分布。
Mol Biol Cell. 2009 Jul;20(13):3064-76. doi: 10.1091/mbc.e08-12-1223. Epub 2009 May 13.
引用本文的文献
1
Sae2 controls Mre11 endo- and exonuclease activities by different mechanisms.Sae2 通过不同的机制控制 Mre11 的内切和外切酶活性。
Nat Commun. 2024 Aug 22;15(1):7221. doi: 10.1038/s41467-024-51493-5.
2
Proteasome activity modulates amyloid toxicity.蛋白酶体活性调节淀粉样毒性。
FEMS Yeast Res. 2022 Mar 9;22(1). doi: 10.1093/femsyr/foac004.
3
The synaptonemal complex central region modulates crossover pathways and feedback control of meiotic double-strand break formation.联会复合体中心区域调节交叉途径和减数分裂双链断裂形成的反馈控制。
Nucleic Acids Res. 2021 Jul 21;49(13):7537-7553. doi: 10.1093/nar/gkab566.
4
DNA-driven condensation assembles the meiotic DNA break machinery.DNA 驱动的凝聚组装了减数分裂 DNA 断裂机制。
Nature. 2021 Apr;592(7852):144-149. doi: 10.1038/s41586-021-03374-w. Epub 2021 Mar 17.
5
Structural and functional characterization of the Spo11 core complex.Spo11 核心复合物的结构与功能特征分析。
Nat Struct Mol Biol. 2021 Jan;28(1):92-102. doi: 10.1038/s41594-020-00534-w. Epub 2021 Jan 4.
6
Multilayered mechanisms ensure that short chromosomes recombine in meiosis.多层次的机制确保了短染色体在减数分裂中进行重组。
Nature. 2020 Jun;582(7810):124-128. doi: 10.1038/s41586-020-2248-2. Epub 2020 May 6.
7
Regulatory control of DNA end resection by Sae2 phosphorylation.Sae2 磷酸化对 DNA 末端切除的调控。
Nat Commun. 2018 Oct 1;9(1):4016. doi: 10.1038/s41467-018-06417-5.
8
Nucleosomes and DNA methylation shape meiotic DSB frequency in transposons and gene regulatory regions.核小体和 DNA 甲基化塑造了减数分裂中转座子和基因调控区的 DSB 频率。
Genome Res. 2018 Apr;28(4):532-546. doi: 10.1101/gr.225599.117. Epub 2018 Mar 12.
9
PRDM9 and Its Role in Genetic Recombination.PRDM9 及其在基因重组中的作用。
Trends Genet. 2018 Apr;34(4):291-300. doi: 10.1016/j.tig.2017.12.017. Epub 2018 Jan 21.
10
The histone variant H2A.Z promotes initiation of meiotic recombination in fission yeast.组蛋白变体 H2A.Z 促进了裂殖酵母中减数分裂重组的起始。
Nucleic Acids Res. 2018 Jan 25;46(2):609-620. doi: 10.1093/nar/gkx1110.
本文引用的文献
1
Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae.酿酒酵母中减数分裂DNA双链断裂形成所需的Mei4、Rec114与其他蛋白质之间的相互作用。
Chromosoma. 2007 Oct;116(5):471-86. doi: 10.1007/s00412-007-0111-y. Epub 2007 Jun 9.
2
Clarifying the mechanics of DNA strand exchange in meiotic recombination.阐明减数分裂重组中DNA链交换的机制。
Nature. 2006 Jul 13;442(7099):153-8. doi: 10.1038/nature04885.
3
Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation.酿酒酵母Mer2、Mei4和Rec114形成一个减数分裂双链断裂形成所必需的复合物。
Genetics. 2006 Aug;173(4):1969-81. doi: 10.1534/genetics.106.058768. Epub 2006 Jun 18.
4
Endonucleolytic processing of covalent protein-linked DNA double-strand breaks.共价蛋白连接的DNA双链断裂的核酸内切加工
Nature. 2005 Aug 18;436(7053):1053-7. doi: 10.1038/nature03872.
5
Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism.抗病毒蛋白Ski8是Spo11在减数分裂DNA断裂形成过程中的直接伙伴，与其在RNA代谢中的细胞质作用无关。
Mol Cell. 2004 Feb 27;13(4):549-59. doi: 10.1016/s1097-2765(04)00063-2.
6
Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation.鉴定酵母Spo11p中对减数分裂DNA双链断裂形成至关重要的残基。
Mol Cell Biol. 2002 Feb;22(4):1106-15. doi: 10.1128/MCB.22.4.1106-1115.2002.
7
Mechanism and control of meiotic recombination initiation.减数分裂重组起始的机制与调控
Curr Top Dev Biol. 2001;52:1-53. doi: 10.1016/s0070-2153(01)52008-6.
8
An atypical topoisomerase II from Archaea with implications for meiotic recombination.一种来自古细菌的非典型拓扑异构酶II及其在减数分裂重组中的意义。
Nature. 1997 Mar 27;386(6623):414-7. doi: 10.1038/386414a0.
Zotero 插件