Serke S, Serke M, Brudler O
J Immunol Methods. 1987 May 20;99(2):167-72. doi: 10.1016/0022-1759(87)90122-0.
Using a two-colour immunofluorescence technique, we have investigated the mitogenic effects of phytohaemagglutinin-M (PHA-M) and of pokeweed nitrogen (PWM) on human lymphocyte subsets. These were identified by CD1, CD3, CD4, CD8, and CD16 monoclonal antibodies, and proliferation was demonstrated by a polyclonal anti-transferrin antibody. Evidence has been obtained for the generation of a population expressing both the CD4 and CD8 antigens simultaneously, in short-term cultures of peripheral blood mononuclear cells in the presence of PWM and of PHA-M.
我们运用双色免疫荧光技术，研究了植物血凝素-M（PHA-M）和商陆氮（PWM）对人淋巴细胞亚群的促有丝分裂作用。这些淋巴细胞亚群通过CD1、CD3、CD4、CD8和CD16单克隆抗体进行鉴定，增殖情况则通过多克隆抗转铁蛋白抗体来证明。在存在PWM和PHA-M的情况下，对外周血单个核细胞进行短期培养，已获得证据表明会产生同时表达CD4和CD8抗原的细胞群体。
