Bach M K, Brashler J R, Gorman R R
Prostaglandins. 1977 Jul;14(1):21-38. doi: 10.1016/0090-6980(77)90154-x.
The mononuclear cells in peritoneal washings from normal rats can be induced to produce large amounts of slow reacting substance of anaphylaxis by incubation with 10 mM cysteine in the presence of the calcium ionophore A-23187. This production of slow reacting substance could be inhibited by the addition of non-steroidal anti-inflammatory drugs, e.g., indomethacin, ibuprofen and flurbiprofen, Furthermore, mediator production was inhibited by eicosatetraynoic acid, the substrate analog of arachidonic acid, and by 9,11-azoprosta-5, 13-dienoic acid (AZO analog 1), a structural analog of the prostaglandin endoperoxide, PGH2, which known to inhibit thromboxane synthesis. Relatively high concentrations of hydrocortisone acetate inhibited mediator production; this inhibition could be partly reversed by the addition of arachidonic acid or to a lesser extent by eicosatrienoic acid. Preliminary results suggest that a small fraction of the 3H-labled arachidonic acid which was taken up by these cells in vitro was associated with slow reacting substance. We postulate that slow reacting substance of anaphylaxis may be derived from a prostaglandin endoperoxide which is formed during the oxidation of arachidonic acid by the prostaglandin fatty acid cyclooxygenase.
在钙离子载体A - 23187存在的情况下，将正常大鼠腹腔灌洗液中的单核细胞与10 mM半胱氨酸一起孵育，可诱导其产生大量过敏反应慢反应物质。添加非甾体抗炎药，如吲哚美辛、布洛芬和氟比洛芬，可抑制这种慢反应物质的产生。此外，花生四烯酸的底物类似物二十碳四炔酸以及前列腺素内过氧化物PGH2的结构类似物9,11 - 偶氮前列腺素 - 5,13 - 二烯酸（AZO类似物1）也可抑制介质的产生，已知后者可抑制血栓素的合成。相对高浓度的醋酸氢化可的松可抑制介质的产生；添加花生四烯酸可部分逆转这种抑制作用，添加二十碳三烯酸则在较小程度上有此作用。初步结果表明，这些细胞在体外摄取的一小部分3H标记花生四烯酸与慢反应物质有关。我们推测，过敏反应慢反应物质可能源自花生四烯酸在前列腺素脂肪酸环氧化酶氧化过程中形成的前列腺素内过氧化物。
