Wang Xiaoqin, Yan Dong, Chen Ling
Key Laboratory of Exploitation and Utilization of Traditional Chinese Medicine Resources of Mianyang, Mianyang, Sichuan, China.
School of Pharmacy, Sichuan College of Traditional Chinese Medicine, Mianyang, Sichuan, China.
PeerJ. 2024 Oct 28;12:e18322. doi: 10.7717/peerj.18322. eCollection 2024.
stands as one of the most extensively utilized traditional Chinese medicinal herbs, with triterpenoids and their derivatives serving as its primary medicinal components. Oxido squalene cyclase (OSC), serving as a crucial enzyme in the triterpenoid synthesis pathway, has the capability to enzymatically generate significant quantities of sterols and triterpenoid intermediates. While the gene family has been identified in numerous species, bioinformatics research on this family remains scant. Presently, the specific members of this gene family in have yet to be definitively determined. In this study, we successfully identified a total of 15 genes within the genome of by conducting homology comparisons. These genes were discovered to be unevenly distributed across the five chromosomes of the species, organized in the form of gene clusters. Subsequently, we conducted a thorough analysis of the gene family's evolutionary relationship by constructing a phylogenetic tree. Other characteristics of family members, including gene structure, conserved motifs, protein three-dimensional structure, subcellular localization, and cis-acting elements were thoroughly characterized. Furthermore, We analyzed the expression of gene in different tissues of by qRT-PCR, and found that the expression of genes in root was higher than that in stem and leaf. Upon comparing the effects of salt, heat, and drought treatments, we observed a significant induction of gene expression in specifically under salt stress conditions. In summary, this study comprehensively identified and analyzed the gene family, aiming to provide basic biological information for exploring the members of gene family.
是最广泛使用的传统中草药之一，三萜类化合物及其衍生物是其主要药用成分。氧化角鲨烯环化酶（OSC）作为三萜类化合物合成途径中的关键酶，能够酶促生成大量的甾醇和三萜类中间体。虽然该基因家族已在许多物种中被鉴定，但对该家族的生物信息学研究仍然很少。目前，该基因家族在中的具体成员尚未确定。在本研究中，我们通过进行同源性比较，在的基因组中成功鉴定出总共15个基因。这些基因被发现不均匀地分布在该物种的五条染色体上，以基因簇的形式组织。随后，我们通过构建系统发育树，对基因家族的进化关系进行了深入分析。对家族成员的其他特征，包括基因结构、保守基序、蛋白质三维结构、亚细胞定位和顺式作用元件进行了全面表征。此外，我们通过qRT-PCR分析了基因在不同组织中的表达，发现基因在根中的表达高于茎和叶。在比较盐、热和干旱处理的影响时，我们观察到在盐胁迫条件下，基因表达在中显著诱导。总之，本研究全面鉴定和分析了基因家族，旨在为探索基因家族的成员提供基础生物学信息。
