Genome-wide identification and expression analysis of the OSC gene family in .

stands as one of the most extensively utilized traditional Chinese medicinal herbs, with triterpenoids and their derivatives serving as its primary medicinal components. Oxido squalene cyclase (OSC), serving as a crucial enzyme in the triterpenoid synthesis pathway, has the capability to enzymatically generate significant quantities of sterols and triterpenoid intermediates. While the gene family has been identified in numerous species, bioinformatics research on this family remains scant. Presently, the specific members of this gene family in have yet to be definitively determined. In this study, we successfully identified a total of 15 genes within the genome of by conducting homology comparisons. These genes were discovered to be unevenly distributed across the five chromosomes of the species, organized in the form of gene clusters. Subsequently, we conducted a thorough analysis of the gene family's evolutionary relationship by constructing a phylogenetic tree. Other characteristics of family members, including gene structure, conserved motifs, protein three-dimensional structure, subcellular localization, and cis-acting elements were thoroughly characterized. Furthermore, We analyzed the expression of gene in different tissues of by qRT-PCR, and found that the expression of genes in root was higher than that in stem and leaf. Upon comparing the effects of salt, heat, and drought treatments, we observed a significant induction of gene expression in specifically under salt stress conditions. In summary, this study comprehensively identified and analyzed the gene family, aiming to provide basic biological information for exploring the members of gene family.