CSIRO Livestock Industries, Queensland Bioscience Precinct, St Lucia, QLD, Australia.
Biomacromolecules. 2009 Nov 9;10(11):3009-14. doi: 10.1021/bm900601h.
Two novel recombinant proteins An16 and Dros16 have recently been generated. These recombinant proteins contain, respectively, sixteen copies of an 11 amino acid repetitive domain (AQTPSSQYGAP) observed in a resilin-like gene from Anopheles gambiae and sixteen copies of a 15 amino acid repetitive domain (GGRPSDSYGAPGGGN) observed in the first exon of the Drosophila melanogaster CG15920 gene. We compare structural characteristics of the proteins and material properties of resulting biopolymers relative to Rec1-resilin, a previously characterized resilin-like protein encoded by the first exon of the Drosophila melanogaster CG15920 gene. While the repetitive domains of natural resilins display significant variation both in terms of amino acid sequence and length, our synthetic polypeptides have been designed as perfect repeats. Using techniques including circular dichroism, atomic force microscopy, and tensile testing, we demonstrate that both An16 and Dros16 have similar material properties to those previously observed in insect and recombinant resilins. Modulus, elasticity, resilience, and dityrosine content in the cross-linked biomaterials were assessed. Despite the reduced complexity of the An16 and Dros16 proteins compared to natural resilins, we have been able to produce elastic and resilient biomaterials with similar properties to resilin.
最近生成了两种新型重组蛋白 An16 和 Dros16。这些重组蛋白分别包含在疟蚊 resilin-like 基因中观察到的 11 个氨基酸重复结构域（AQTPSSQYGAP）的 16 个拷贝和在果蝇 CG15920 基因第一个外显子中观察到的 15 个氨基酸重复结构域（GGRPSDSYGAPGGGN）的 16 个拷贝。我们比较了蛋白质的结构特征和所得生物聚合物的材料特性与 Rec1-resilin 的差异，Rec1-resilin 是由果蝇 CG15920 基因第一个外显子编码的先前表征的 resilin-like 蛋白。虽然天然 resilin 的重复结构域在氨基酸序列和长度方面都存在显著差异，但我们设计的合成多肽是完美的重复。通过使用包括圆二色性、原子力显微镜和拉伸测试在内的技术，我们证明了 An16 和 Dros16 都具有与以前在昆虫和重组 resilin 中观察到的相似的材料特性。评估了交联生物材料的模量、弹性、弹性恢复率和二酪氨酸含量。尽管与天然 resilin 相比，An16 和 Dros16 蛋白的复杂性降低，但我们已经能够生产出具有类似 resilin 特性的弹性和弹性生物材料。
