College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Cell Stress Chaperones. 2010 May;15(3):335-42. doi: 10.1007/s12192-009-0148-3. Epub 2009 Oct 15.
The objective of this study is to investigate the expression and distribution of heat shock protein 70 (Hsp70) in the intestine of intrauterine growth retardation (IUGR) piglets. Samples from the duodenum, prejejunum, distal jejunum, ileum, and colon of IUGR and normal-body-weight (NBW) piglets were collected at birth. The results indicated that the body and intestine weight of IUGR piglets were significantly lower than NBW piglets. The villus height and villus/crypt ratio in jejunum and ileum of IUGR piglets were significantly reduced compared to NBW piglets. These results indicated that IUGR causes abnormal gastrointestinal morphologies and gastrointestinal dysfunction. The mRNA of hsp70 was increased in prejejunum (P < 0.05), distal jejunum (P < 0.05), and colon in IUGR piglets. However, the hsp70 mRNA in ileum of piglets with IUGR was decreased. Similar to hsp70 mRNA, the protein levels of Hsp70 in prejejunum (P < 0.05), distal jejunum, and colon (P < 0.05) in IUGR piglets were higher than those in NBW piglets. These results indicated that the expression of Hsp70 in the intestinal piglets was upregulated by IUGR, and different intestinal sites had different responses to stress. Meanwhile, the localization of Hsp70 in the epithelial cells of the whole villi and intestinal gland rather than in the lamina propria and myenteron suggested that Hsp70 has a cytoprotective role in epithelial cell function and structure.
本研究旨在探讨热休克蛋白 70（Hsp70）在宫内生长迟缓（IUGR）仔猪肠道中的表达和分布。在出生时收集了 IUGR 和正常体重（NBW）仔猪十二指肠、空肠前段、空肠远端、回肠和结肠的样本。结果表明，IUGR 仔猪的体质量和肠道重量明显低于 NBW 仔猪。与 NBW 仔猪相比，IUGR 仔猪空肠和回肠的绒毛高度和绒毛/隐窝比值显著降低。这些结果表明，IUGR 导致胃肠道形态异常和胃肠道功能障碍。Hsp70 的 mRNA 在 IUGR 仔猪的空肠前段（P < 0.05）、空肠远端（P < 0.05）和结肠中增加。然而，IUGR 仔猪回肠中的 hsp70 mRNA 减少。与 hsp70 mRNA 相似，Hsp70 蛋白水平在 IUGR 仔猪的空肠前段（P < 0.05）、空肠远端和结肠（P < 0.05）中高于 NBW 仔猪。这些结果表明，IUGR 上调了肠道仔猪 Hsp70 的表达，不同的肠道部位对应激有不同的反应。同时，Hsp70 在整个绒毛和肠腺上皮细胞中的定位而不是在固有层和肌间神经丛中，表明 Hsp70 在维持上皮细胞功能和结构方面具有细胞保护作用。
