Bioactive Products Engineering Research Center for Gansu Distinctive Plants, Northwest Normal University, Lanzhou 730070, China.
Int J Biol Macromol. 2010 Jan 1;46(1):59-66. doi: 10.1016/j.ijbiomac.2009.10.004. Epub 2009 Oct 28.
Sulfated polysaccharides exerted potential biological property which was relative to degree of sulfation (DS), M(w), substitution position and chain conformation. In the present study, commercial guar gum was purified and its sulfated derivates with different DS and M(w) were synthesized. FT-IR and 13C NMR analysis indicated that C-6 substitution was predominant in sulfated samples compared with other positions. In the sulfation reaction, a sharp decrease in M(w) was observed. The d(f) values from 1.92 to 2.85 indicated that the -SO3H groups led to the relatively expanded conformation of sulfated polysaccharides. Antioxidant assays showed that sulfated polysaccharides had better antioxidant activities. The data obtained in in vitro models indicated that high DS and low M(w) showed the best antioxidant capacities.
硫酸化多糖具有与硫酸化程度（DS）、Mw、取代位置和链构象相关的潜在生物学特性。在本研究中，对商业瓜尔胶进行了纯化，并合成了具有不同 DS 和 Mw 的硫酸化衍生物。FT-IR 和 13C NMR 分析表明，与其他位置相比，硫酸化样品中主要发生 C-6 取代。在硫酸化反应中，Mw 急剧下降。d(f)值为 1.92 至 2.85，表明 -SO3H 基团导致硫酸化多糖具有相对扩展的构象。抗氧化测定表明，硫酸化多糖具有更好的抗氧化活性。体外模型中获得的数据表明，高 DS 和低 Mw 表现出最佳的抗氧化能力。
