Laboratório de Biotecnologia de Polímeros Naturais (), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte, 59078-970, Brazil.
Programa de Pós-graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte, 59012-570, Brazil.
Mar Drugs. 2019 Feb 9;17(2):105. doi: 10.3390/md17020105.
Green seaweeds are rich sources of sulfated polysaccharides (SPs) with potential biomedical and nutraceutical applications. The aim of this work was to evaluate the immunostimulatory activity of SPs from the seaweed, var on murine RAW 264.7 macrophages. SPs were evaluated for their ability to modify cell viability and to stimulate the production of inflammatory mediators, such as nitric oxide (NO), intracellular reactive oxygen species (ROS), and cytokines. Additionally, their effect on inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) gene expression was investigated. The results showed that SPs were not cytotoxic and were able to increase in the production of NO, ROS and the cytokines, tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6). It was also observed that treatment with SPs increased iNOS and COX-2 gene expression. Together, these results indicate that var. SPs have strong immunostimulatory activity, with potential biomedical applications.
绿藻是富含硫酸多糖（SPs）的资源，具有潜在的生物医学和营养应用。本工作旨在评估 SPs 对鼠源 RAW 264.7 巨噬细胞的免疫刺激活性。评估了 SPs 对细胞活力的改变能力，并刺激产生炎症介质，如一氧化氮（NO）、细胞内活性氧物种（ROS）和细胞因子。此外，还研究了它们对诱导型一氧化氮合酶（iNOS）和环氧化酶 2（COX-2）基因表达的影响。结果表明，SPs 无细胞毒性，能够增加 NO、ROS 和细胞因子（肿瘤坏死因子-α（TNF-α）和白细胞介素 6（IL-6））的产生。还观察到，SPs 处理增加了 iNOS 和 COX-2 基因表达。总之，这些结果表明，var. SPs 具有很强的免疫刺激活性，具有潜在的生物医学应用。
