Lin Shih-Yao, Hameed Asif, Hung Mei-Hua, Liu You-Cheng, Hsu Yi-Han, Young Li-Sen, Young Chiu-Chung
Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, Yunlin, Taiwan.
Int J Syst Evol Microbiol. 2015 Mar;65(Pt 3):902-909. doi: 10.1099/ijs.0.000035. Epub 2014 Dec 18.
An aerobic, Gram-stain-negative, rod-shaped and polar-flagellated bacterium, designated strain CC-MHH0089(T), was isolated from a soil sample taken on Matsu Island (Taiwan). Strain CC-MHH0089(T) grew at 15-30 °C and pH 5.0-10.0 and tolerated ≤8 % (w/v) NaCl. 16S rRNA gene sequence analysis showed high pairwise sequence similarity to Pseudomonas azotifigens 6H33b(T) (97.3 %) and Pseudomonas balearica SP1402(T) (96.7 %) and lower sequence similarity to other strains (<96.0 %). In DNA-DNA reassociation experiments, the relatedness of strain CC-MHH0089(T) to P. azotifigens JCM 12708(T) was 38.3 % (reciprocal value 19.5 %). Evolutionary trees reconstructed on the basis of 16S rRNA, gyrB and rpoB gene sequences revealed a varying phylogenetic neighbourhood of strain CC-MHH0089(T) with regard to the most closely related type strains. The predominant quinone system was ubiquinone 9 (Q-9) and the DNA G+C content was 63.6 mol%. The major fatty acids were C12 : 0, C16 : 0, C17 : 0, C19 : 0 cyclo ω8c and summed features 2 (C14 : 0 3-OH/iso-C16 : 1 I), 3 (C16 : 1ω7c/C16 : 1ω6c) and 8 (C18 : 1ω7c/C18 : 1ω6c). The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. According to its distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-MHH0089(T) is proposed to represent a novel species within the genus Pseudomonas, for which the name Pseudomonas matsuisoli sp. nov. is proposed. The type strain is CC-MHH0089(T) ( = BCRC 80771(T) = JCM 30078(T)).
从台湾马祖岛采集的土壤样本中分离出一株需氧、革兰氏染色阴性、杆状且具极生鞭毛的细菌，命名为菌株CC-MHH0089(T)。菌株CC-MHH0089(T)在15 - 30℃和pH 5.0 - 10.0条件下生长，耐受≤8%（w/v）的NaCl。16S rRNA基因序列分析表明，其与固氮假单胞菌6H33b(T)（97.3%）和巴利阿里假单胞菌SP1402(T)（96.7%）的序列相似性较高，与其他菌株的序列相似性较低（<96.0%）。在DNA - DNA重杂交实验中，菌株CC-MHH0089(T)与固氮假单胞菌JCM 12708(T)的相关性为38.3%（ reciprocal value 19.5%）。基于16S rRNA、gyrB和rpoB基因序列重建的进化树显示，菌株CC-MHH0089(T)与其最密切相关的模式菌株的系统发育邻域不同。主要醌系统为泛醌9（Q - 9），DNA G + C含量为63.6 mol%。主要脂肪酸为C12 : 0、C16 : 0、C17 : 0、C19 : 0环ω8c以及总和特征2（C14 : 0 3 - OH/异C16 : 1 I）、3（C16 : 1ω7c/C16 : 1ω6c）和8（C18 : 1ω7c/C18 : 1ω6c）。主要极性脂为磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺和二磷脂酰甘油。根据其独特的系统发育、表型和化学分类特征，菌株CC-MHH0089(T)被提议代表假单胞菌属内的一个新物种，为此提出新名称马祖假单胞菌（Pseudomonas matsuisoli sp. nov.）。模式菌株为CC-MHH0089(T)（= BCRC 80771(T)= JCM 30078(T)）。
