Health Protection Agency, North West Local and Regional Services, Liverpool, UK.
Appl Environ Microbiol. 2010 Jan;76(1):129-35. doi: 10.1128/AEM.00942-09. Epub 2009 Oct 23.
This study uses multilocus sequence typing (MLST) to investigate the epidemiology of Campylobacter coli in a continuous study of a population in Northwest England. All cases of Campylobacter identified in four Local Authorities (government administrative boundaries) between 2003 and 2006 were identified to species level and then typed, using MLST. Epidemiological information was collected for each of these cases, including food and recreational exposure variables, and the epidemiologies of C. jejuni and C. coli were compared using case-case methodology. Samples of surface water thought to represent possible points of exposure to the populations under study were also sampled, and campylobacters were typed with multilocus sequence typing. Patients with C. coli were more likely to be older and female than patients with C. jejuni. In logistic regression, C. coli infection was positively associated with patients eating undercooked eggs, eating out, and reporting problems with their water supply prior to illness. C. coli was less associated with consuming pork products. Most of the cases of C. coli yielded sequence types described elsewhere in both livestock and poultry, but several new sequence types were also identified in human cases and water samples. There was no overlap between types identified in humans and surface waters, and genetic analysis suggested three distinct clades but with several "intermediate" types from water that were convergent with the human clade. There is little evidence to suggest that epidemiological differences between human cases of C. coli and C. jejuni are a result of different food or behavioral exposures alone.
本研究采用多位点序列分型（MLST）方法，对英格兰西北部一个人群进行的连续研究中，对空肠弯曲菌进行了流行病学研究。在 2003 年至 2006 年期间，在四个地方当局（政府行政边界）中确定了所有鉴定的弯曲菌属病例的物种水平，然后使用 MLST 进行分型。对这些病例中的每一个都收集了流行病学信息，包括食物和娱乐暴露变量，并使用病例对照方法比较了空肠弯曲菌和结肠弯曲菌的流行病学。还对可能代表研究人群暴露点的地表水样本进行了采样，并使用多位点序列分型对弯曲菌进行了分型。与空肠弯曲菌患者相比，结肠弯曲菌患者年龄更大，女性更多。在逻辑回归中，结肠弯曲菌感染与患者食用未煮熟的鸡蛋、外出就餐以及在发病前报告供水问题呈正相关。结肠弯曲菌与食用猪肉产品的相关性较低。大多数结肠弯曲菌病例的序列类型与家畜和家禽中的其他地方描述的序列类型相同，但在人类病例和水样中也发现了几种新的序列类型。在人类和地表水之间没有鉴定出相同的类型，遗传分析表明存在三个不同的分支，但有几种来自水的“中间”类型与人类分支趋同。几乎没有证据表明空肠弯曲菌和结肠弯曲菌的人类病例之间的流行病学差异是由不同的食物或行为暴露单独引起的。
