Chmaisse Hania Nakkash, Jammal Manal, Fakhoury Hana, Fakhoury Rajaa
College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
Saudi J Kidney Dis Transpl. 2009 Nov;20(6):1038-46.
Type-2 diabetes mellitus (T2DM) is a chronic disorder characterized by a varying range of predominant insulin resistance with relative insulin deficiency, to predominant insulin secretory defect with or without insulin resistance. Familial clustering as well as epidemiological studies has shown that genetic factors play a role in the development and progression of the disease. Among the genetic factors found to be associated with development of T2DM is the angiotensin-I converting enzyme (ACE) gene, which is located on chromosome 17q23. This study was conducted to study the association between ACE gene insertion/deletion (I/D) polymorphism and T2DM in a Lebanese diabetic cohort. Fifty-one patients with T2DM and 40 control subjects from different parts of Lebanon underwent genotyping for the ACE I/D, which was performed by PCR using specific primers. Chi-square and analysis of variance (ANOVA) were used for association studies and to assess the differences in the values among the groups. The distribution of the genotypes in the patients was as follows: 15/51 (29.4%) were homozygous for deletion allele (DD genotype), 24/51 (47.1%) were heterozygous (ID genotype), and 12/51 (23.5%) were homozygous for insertion allele (II genotype). Among the control subjects, 16/40 (40%) were homozygous for deletion (DD genotype), 13/40 (32.5%) were heterozygous (ID genotype), and 11/40 (27.5%) were homozygous for insertion (II genotype). The prevalence of the D-allele in T2DM patients (52.9%) was not significantly different from that in the controls (56.3%). Thus, ACE I/D dimorphism cannot be considered a risk factor for T2DM in the Lebanese population.
2型糖尿病（T2DM）是一种慢性疾病，其特征是存在不同程度的以相对胰岛素缺乏为主的胰岛素抵抗，到以胰岛素分泌缺陷为主且伴有或不伴有胰岛素抵抗。家族聚集性以及流行病学研究表明，遗传因素在该疾病的发生和发展中起作用。在已发现的与T2DM发生相关的遗传因素中，有位于17号染色体q23区域的血管紧张素-I转换酶（ACE）基因。本研究旨在探讨黎巴嫩糖尿病队列中ACE基因插入/缺失（I/D）多态性与T2DM之间的关联。来自黎巴嫩不同地区的51例T2DM患者和40例对照者接受了ACE I/D基因分型，采用特异性引物通过聚合酶链反应（PCR）进行。卡方检验和方差分析（ANOVA）用于关联研究，并评估各组间数值的差异。患者中基因型的分布如下：15/51（29.4%）为缺失等位基因纯合子（DD基因型），24/51（47.1%）为杂合子（ID基因型），12/51（23.5%）为插入等位基因纯合子（II基因型）。在对照者中，16/40（40%）为缺失纯合子（DD基因型），13/40（32.5%）为杂合子（ID基因型），11/40（27.5%）为插入纯合子（II基因型）。T2DM患者中D等位基因的患病率（52.9%）与对照组（56.3%）无显著差异。因此，在黎巴嫩人群中，ACE I/D二态性不能被视为T2DM的危险因素。
