Karlsson R, Aspenström P, Byström A S
Department of Zoological Cell Biology, University of Stockholm, Sweden.
Mol Cell Biol. 1991 Jan;11(1):213-7. doi: 10.1128/mcb.11.1.213-217.1991.
Recently it was demonstrated that beta-actin can be produced in Saccharomyces cerevisiae by using the expression plasmid pY beta actin (R. Karlsson, Gene 68:249-258, 1988), and several site-specific mutants are now being produced in a protein engineering study. To establish a system with which recombinant actin mutants can be tested in vivo and thus enable a correlation to be made with functional effects observed in vitro, a yeast strain lacking endogenous yeast actin and expressing exclusively beta-actin was constructed. This strain is viable but has an altered morphology and a slow-growth phenotype and is temperature sensitive to the point of lethality at 37 degrees C.
最近有研究表明，利用表达质粒pYβ肌动蛋白可在酿酒酵母中产生β肌动蛋白（R. 卡尔松，《基因》68:249 - 258，1988），目前在一项蛋白质工程研究中正在产生几种位点特异性突变体。为了建立一个能够在体内测试重组肌动蛋白突变体并因此能够将其与体外观察到的功能效应建立关联的系统，构建了一种缺乏内源性酵母肌动蛋白且仅表达β肌动蛋白的酵母菌株。该菌株能够存活，但形态发生了改变，具有生长缓慢的表型，并且对温度敏感，在37摄氏度时会达到致死点。
