Department of Medicine, The University of Chicago, Chicago.
J Exp Med. 1934 Jan 1;59(1):75-82. doi: 10.1084/jem.59.1.75.
The alcohol-insoluble polysaccharides of gonococcus and meningococcus were found to contain 4.2 and 3.7 per cent nitrogen respectively, to be protein-free by chemical test, to reduce FehIing-Benedict solution only after hydrolysis. They were non-toxic for rabbits and mice, and failed to engender antibodies (precipitins) in rabbits. They produced no cutaneous reactions in normal, snuffle-free rabbits, but caused typical allergic reactions of the delayed type in rabbits rendered hypersensitive to these organisms. Both carbohydrates reacted in high dilution with Antipneumococcus Serum Type III. For comparison, carbohydrates were prepared also from Micrococcus catarrhalis, Streptococcus hemolyticus, Staphylococcus aureus, and a rough strain of pneumococcus.
淋病奈瑟球菌和脑膜炎奈瑟球菌的醇不溶性多糖分别含有 4.2%和 3.7%的氮，经化学试验证明不含蛋白质，只有水解后才能还原费林-本尼迪克特溶液。它们对兔和鼠均无毒，不能在兔体内产生抗体（沉淀素）。在正常、无鼻液的兔体内，它们不能引起皮肤反应，但能使对这些菌敏感的兔产生典型的迟发型过敏反应。这两种碳水化合物都与抗肺炎球菌血清Ⅲ型发生高稀释反应。为了比较，还从卡他莫拉菌、溶血性链球菌、金黄色葡萄球菌和粗糙型肺炎球菌中制备了碳水化合物。
