宿主年龄和孵化温度对鸡胚感染水疱性口炎病毒的影响。
THE INFLUENCE OF AGE OF HOST AND TEMPERATURE OF INCUBATION ON INFECTION OF THE CHICK EMBRYO WITH VESICULAR STOMATITIS VIRUS.
机构信息
Department of Animal and Plant Pathology of The Rockefeller Institute for Medical Research, Princeton, New Jersey.
出版信息
J Exp Med. 1943 Jul 1;78(1):17-26. doi: 10.1084/jem.78.1.17.
Chick embryos after 7 days of incubation were found to be much more susceptible to infection with vesicular stomatitis virus than were 10 day embryos. They had a 100 per cent mortality and were very suitable for titrations of the virus. The rate of increase of virus in 7 and 10 day embryos was studied. Two different temperatures of incubation were employed, 35-36 degrees C. and 39-40 degrees C., and the growth curves for the virus under the different conditions are presented. 10 day embryos were highly resistant and at 39-40 degrees C. more than half of them survived. At the lower temperature of incubation, 35-36 degrees C., all 10 day embryos died, but they survived much longer than did 7 day embryos. In the 7 day embryos death occurred after about 12 hours at 39-40 degrees C. and after about 16 hours at 35-36 degrees C., or earlier at the higher temperature. In embryos of both ages the virus titer reached at the higher temperature was only about 1 per cent of that reached at 35-36 degrees C., even in those that died.
研究发现，与 10 日龄鸡胚相比，孵育 7 天的鸡胚对水疱性口炎病毒的易感性更高。这些鸡胚的死亡率为 100%，非常适合用于病毒滴定。研究了在 7 日龄和 10 日龄鸡胚中病毒的增殖速度。采用了两种不同的孵育温度，35-36°C 和 39-40°C，并呈现了在不同条件下病毒的生长曲线。10 日龄鸡胚具有高度抗性，在 39-40°C 下，超过一半的鸡胚存活。在较低的孵育温度 35-36°C 下，所有 10 日龄鸡胚均死亡，但它们的存活时间比 7 日龄鸡胚长得多。在 7 日龄鸡胚中，在 39-40°C 下，鸡胚在大约 12 小时后死亡，在 35-36°C 下，大约 16 小时后死亡，或者在较高温度下更早死亡。在两个年龄组的鸡胚中，在较高温度下达到的病毒滴度仅为在 35-36°C 下达到的病毒滴度的约 1%，即使在那些死亡的鸡胚中也是如此。
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