Xerri L, Hassoun J, Planche J, Guigou V, Grob J J, Parc P, Birnbaum D, deLapeyriere O
Laboratoire d'Anatomo-pathologie, Institut Paoli-Calmettes, U119 INSERM, Marseille, France.
Am J Pathol. 1991 Jan;138(1):9-15.
Biopsy samples from five acquired immune deficiency syndrome (AIDS)-Kaposi's sarcomas and one non-AIDS-associated Kaposi's sarcoma were assayed by in situ RNA hybridization onto paraformaldehyde-fixed, paraffin-embedded skin sections for the presence of two fibroblast growth factor gene transcripts, FGFB and FGF5. FGF5 gene expression was detected in the characteristic Kaposi's sarcoma spindle-shaped cells in the five samples from human immunodeficiency-positive (HIV+) patients. FGFB transcripts were detected in Kaposi's sarcoma cells as well as in epidermis of HIV- and HIV+ patients. These results complement the observations about growth factor gene expression done on Kaposi's sarcoma-derived cell lines, which thus appear to be representative of what happens in vivo. Furthermore, they demonstrate a contrasting expression pattern of FGF5 and FGFB genes, both involved in the growth factor pathogenic cascade leading to Kaposi's sarcoma.
通过原位RNA杂交技术，对来自5例获得性免疫缺陷综合征（AIDS）-卡波西肉瘤和1例非AIDS相关卡波西肉瘤的活检样本进行检测，这些样本为经多聚甲醛固定、石蜡包埋的皮肤切片，目的是检测两种成纤维细胞生长因子基因转录本FGFB和FGF5的存在情况。在来自人类免疫缺陷病毒阳性（HIV+）患者的5个样本中，在典型的卡波西肉瘤梭形细胞中检测到了FGF5基因表达。在卡波西肉瘤细胞以及HIV阴性和HIV阳性患者的表皮中均检测到了FGFB转录本。这些结果补充了对卡波西肉瘤衍生细胞系进行的生长因子基因表达观察结果，因此这些细胞系似乎代表了体内发生的情况。此外，它们证明了FGF5和FGFB基因的表达模式形成对比，这两种基因均参与导致卡波西肉瘤的生长因子致病级联反应。
