利用麦芽糖增加谷氨酸棒杆菌的葡萄糖利用率及其在 L-缬氨酸生产中的应用。
Increased glucose utilization in Corynebacterium glutamicum by use of maltose, and its application for the improvement of L-valine productivity.
机构信息
Institute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany.
出版信息
Appl Environ Microbiol. 2010 Jan;76(1):370-4. doi: 10.1128/AEM.01553-09. Epub 2009 Oct 30.
Abstract
Corynebacterium glutamicum efficiently utilizes maltose as a substrate. We show here that the presence of maltose increases glucose utilization by raising the expression of ptsG, which encodes the glucose-specific EII permease of the phosphotransferase system. Consequently, the L-valine productivity of a pyruvate dehydrogenase complex-deficient C. glutamicum strain was improved by the presence of maltose.
摘要
谷氨酸棒杆菌能够有效地利用麦芽糖作为底物。我们在这里表明，麦芽糖的存在通过提高编码磷酸转移酶系统中葡萄糖特异性 EII 通透酶的 ptsG 的表达来增加葡萄糖的利用。因此，麦芽糖的存在提高了丙酮酸脱氢酶复合体缺陷型谷氨酸棒杆菌菌株的 L-缬氨酸生产力。
相似文献
1
Increased glucose utilization in Corynebacterium glutamicum by use of maltose, and its application for the improvement of L-valine productivity.利用麦芽糖增加谷氨酸棒杆菌的葡萄糖利用率及其在 L-缬氨酸生产中的应用。
Appl Environ Microbiol. 2010 Jan;76(1):370-4. doi: 10.1128/AEM.01553-09. Epub 2009 Oct 30.
2
Maltose uptake by the novel ABC transport system MusEFGK2I causes increased expression of ptsG in Corynebacterium glutamicum.新型 ABC 转运系统 MusEFGK2I 摄取麦芽糖会导致谷氨酸棒杆菌中 ptsG 的表达增加。
J Bacteriol. 2013 Jun;195(11):2573-84. doi: 10.1128/JB.01629-12. Epub 2013 Mar 29.
3
Platform engineering of Corynebacterium glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of L-lysine, L-valine, and 2-ketoisovalerate.谷氨酸棒杆菌的丙酮酸脱氢酶复合物活性降低的平台工程，用于提高 L-赖氨酸、L-缬氨酸和 2-酮异戊酸的产量。
Appl Environ Microbiol. 2013 Sep;79(18):5566-75. doi: 10.1128/AEM.01741-13. Epub 2013 Jul 8.
4
Impact of CO/HCO Availability on Anaplerotic Flux in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains.CO/HCO 可用性对丙酮酸脱氢酶复合物缺陷型谷氨酸棒状杆菌补料流加的影响。
J Bacteriol. 2019 Sep 20;201(20). doi: 10.1128/JB.00387-19. Print 2019 Oct 15.
5
L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum.利用丙酮酸脱氢酶复合体缺陷型谷氨酸棒杆菌生产L-缬氨酸。
Appl Environ Microbiol. 2007 Apr;73(7):2079-84. doi: 10.1128/AEM.02826-06. Epub 2007 Feb 9.
6
Phosphotransferase system-mediated glucose uptake is repressed in phosphoglucoisomerase-deficient Corynebacterium glutamicum strains.磷酸转移酶系统介导的葡萄糖摄取在磷酸葡糖异构酶缺陷型谷氨酸棒杆菌菌株中受到抑制。
Appl Environ Microbiol. 2013 Apr;79(8):2588-95. doi: 10.1128/AEM.03231-12. Epub 2013 Feb 8.
7
L-valine production during growth of pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum in the presence of ethanol or by inactivation of the transcriptional regulator SugR.在乙醇存在的情况下，通过丙酮酸脱氢酶复合体缺陷型谷氨酸棒杆菌生长过程中产生L-缬氨酸，或通过转录调节因子SugR的失活产生L-缬氨酸。
Appl Environ Microbiol. 2009 Feb;75(4):1197-200. doi: 10.1128/AEM.02351-08. Epub 2008 Dec 16.
8
Analysing overexpression of L-valine biosynthesis genes in pyruvate-dehydrogenase-deficient Corynebacterium glutamicum.分析丙酮酸脱氢酶缺陷型谷氨酸棒杆菌中 L-缬氨酸生物合成基因的过表达。
J Ind Microbiol Biotechnol. 2010 Mar;37(3):263-70. doi: 10.1007/s10295-009-0669-x. Epub 2009 Dec 11.
9
Increased glucose utilization and cell growth of Corynebacterium glutamicum by modifying the glucose-specific phosphotransferase system (PTS) genes.通过修饰葡萄糖特异性磷酸转移酶系统（PTS）基因提高谷氨酸棒杆菌的葡萄糖利用率和细胞生长。
Can J Microbiol. 2016 Dec;62(12):983-992. doi: 10.1139/cjm-2016-0027. Epub 2016 Jul 15.
10
Elongation factor P is required for EII translation in Corynebacterium glutamicum due to an essential polyproline motif.延伸因子 P 由于一个必需的多脯氨酸基序，在谷氨酸棒杆菌中是 EII 翻译所必需的。
Mol Microbiol. 2021 Feb;115(2):320-331. doi: 10.1111/mmi.14618. Epub 2020 Oct 25.
引用本文的文献
1
Transcriptomics of : metabolism patterns and cellular responses under high-density culture conditions.高密度培养条件下的转录组学：代谢模式与细胞反应
Front Bioeng Biotechnol. 2023 Oct 12;11:1274020. doi: 10.3389/fbioe.2023.1274020. eCollection 2023.
2
Handling Several Sugars at a Time: a Case Study of Xyloglucan Utilization by .同时处理多种糖：木葡聚糖利用的案例研究。
mBio. 2021 Dec 21;12(6):e0220621. doi: 10.1128/mBio.02206-21. Epub 2021 Nov 9.
3
Engineering of microbial cells for L-valine production: challenges and opportunities.微生物细胞工程生产 L-缬氨酸：挑战与机遇。
Microb Cell Fact. 2021 Aug 30;20(1):172. doi: 10.1186/s12934-021-01665-5.
4
Metabolic engineering of glucose uptake systems in Corynebacterium glutamicum for improving the efficiency of L-lysine production.在谷氨酸棒杆菌中葡萄糖摄取系统的代谢工程改造，以提高 L-赖氨酸生产效率。
J Ind Microbiol Biotechnol. 2019 Jul;46(7):937-949. doi: 10.1007/s10295-019-02170-w. Epub 2019 Apr 1.
5
Systems metabolic engineering strategies for the production of amino acids.用于氨基酸生产的系统代谢工程策略。
Synth Syst Biotechnol. 2017 Aug 2;2(2):87-96. doi: 10.1016/j.synbio.2017.07.003. eCollection 2017 Jun.
6
Transcription of Sialic Acid Catabolism Genes in Corynebacterium glutamicum Is Subject to Catabolite Repression and Control by the Transcriptional Repressor NanR.谷氨酸棒杆菌中唾液酸分解代谢基因的转录受分解代谢物阻遏作用以及转录阻遏蛋白NanR的调控。
J Bacteriol. 2016 Jul 28;198(16):2204-18. doi: 10.1128/JB.00820-15. Print 2016 Aug 15.
7
Maltose-Dependent Transcriptional Regulation of the mal Regulon by MalR in Streptococcus pneumoniae.肺炎链球菌中MalR对mal操纵子的麦芽糖依赖性转录调控
PLoS One. 2015 Jun 1;10(6):e0127579. doi: 10.1371/journal.pone.0127579. eCollection 2015.
8
The α-glucan phosphorylase MalP of Corynebacterium glutamicum is subject to transcriptional regulation and competitive inhibition by ADP-glucose.谷氨酸棒杆菌的α-葡聚糖磷酸化酶MalP受转录调控以及ADP-葡萄糖的竞争性抑制。
J Bacteriol. 2015 Apr;197(8):1394-407. doi: 10.1128/JB.02395-14. Epub 2015 Feb 9.
9
Metabolic engineering of Corynebacterium glutamicum aimed at alternative carbon sources and new products.靶向替代碳源和新产品的谷氨酸棒杆菌代谢工程。
Comput Struct Biotechnol J. 2012 Oct 30;3:e201210004. doi: 10.5936/csbj.201210004. eCollection 2012.
10
Platform engineering of Corynebacterium glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of L-lysine, L-valine, and 2-ketoisovalerate.谷氨酸棒杆菌的丙酮酸脱氢酶复合物活性降低的平台工程，用于提高 L-赖氨酸、L-缬氨酸和 2-酮异戊酸的产量。
Appl Environ Microbiol. 2013 Sep;79(18):5566-75. doi: 10.1128/AEM.01741-13. Epub 2013 Jul 8.
本文引用的文献
1
Role of the transcriptional regulator RamB (Rv0465c) in the control of the glyoxylate cycle in Mycobacterium tuberculosis.转录调节因子RamB（Rv0465c）在结核分枝杆菌乙醛酸循环调控中的作用。
J Bacteriol. 2009 Dec;191(23):7260-9. doi: 10.1128/JB.01009-09. Epub 2009 Sep 18.
2
Roles of maltodextrin and glycogen phosphorylases in maltose utilization and glycogen metabolism in Corynebacterium glutamicum.麦芽糖糊精和糖原磷酸化酶在谷氨酸棒杆菌利用麦芽糖及糖原代谢中的作用
Microbiology (Reading). 2009 Feb;155(Pt 2):347-358. doi: 10.1099/mic.0.023614-0.
3
L-valine production during growth of pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum in the presence of ethanol or by inactivation of the transcriptional regulator SugR.在乙醇存在的情况下，通过丙酮酸脱氢酶复合体缺陷型谷氨酸棒杆菌生长过程中产生L-缬氨酸，或通过转录调节因子SugR的失活产生L-缬氨酸。
Appl Environ Microbiol. 2009 Feb;75(4):1197-200. doi: 10.1128/AEM.02351-08. Epub 2008 Dec 16.
4
Carbon catabolite repression in bacteria: many ways to make the most out of nutrients.细菌中的碳分解代谢物阻遏：充分利用营养物质的多种方式。
Nat Rev Microbiol. 2008 Aug;6(8):613-24. doi: 10.1038/nrmicro1932.
5
Corynebacterium glutamicum tailored for high-yield L-valine production.经改造用于高产L-缬氨酸生产的谷氨酸棒杆菌。
Appl Microbiol Biotechnol. 2008 Jun;79(3):471-9. doi: 10.1007/s00253-008-1444-z. Epub 2008 Apr 1.
6
The mechanisms of carbon catabolite repression in bacteria.细菌中碳分解代谢物阻遏的机制。
Curr Opin Microbiol. 2008 Apr;11(2):87-93. doi: 10.1016/j.mib.2008.02.007. Epub 2008 Mar 21.
7
Ethanol catabolism in Corynebacterium glutamicum.谷氨酸棒杆菌中的乙醇分解代谢
J Mol Microbiol Biotechnol. 2008;15(4):222-33. doi: 10.1159/000107370. Epub 2007 Aug 13.
8
The alcohol dehydrogenase gene adhA in Corynebacterium glutamicum is subject to carbon catabolite repression.谷氨酸棒杆菌中的乙醇脱氢酶基因adhA受到碳分解代谢物阻遏作用的调控。
J Bacteriol. 2007 Oct;189(20):7408-16. doi: 10.1128/JB.00791-07. Epub 2007 Aug 10.
9
Transcriptionally regulated adhA gene encodes alcohol dehydrogenase required for ethanol and n-propanol utilization in Corynebacterium glutamicum R.转录调控的adhA基因编码谷氨酸棒杆菌R利用乙醇和正丙醇所需的乙醇脱氢酶。
Appl Microbiol Biotechnol. 2007 Oct;76(6):1347-56. doi: 10.1007/s00253-007-1094-6. Epub 2007 Jul 24.
10
L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum.利用丙酮酸脱氢酶复合体缺陷型谷氨酸棒杆菌生产L-缬氨酸。
Appl Environ Microbiol. 2007 Apr;73(7):2079-84. doi: 10.1128/AEM.02826-06. Epub 2007 Feb 9.
Zotero 插件