Ma Qian, Zhang Quanwei, Xu Qingyang, Zhang Chenglin, Li Yanjun, Fan Xiaoguang, Xie Xixian, Chen Ning
National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
Synth Syst Biotechnol. 2017 Aug 2;2(2):87-96. doi: 10.1016/j.synbio.2017.07.003. eCollection 2017 Jun.
Systems metabolic engineering is a multidisciplinary area that integrates systems biology, synthetic biology and evolutionary engineering. It is an efficient approach for strain improvement and process optimization, and has been successfully applied in the microbial production of various chemicals including amino acids. In this review, systems metabolic engineering strategies including pathway-focused approaches, systems biology-based approaches, evolutionary approaches and their applications in two major amino acid producing microorganisms: and are summarized.
系统代谢工程是一个整合了系统生物学、合成生物学和进化工程的多学科领域。它是一种用于菌株改良和工艺优化的有效方法，已成功应用于包括氨基酸在内的各种化学品的微生物生产中。在本综述中，总结了包括以途径为重点的方法、基于系统生物学的方法、进化方法等系统代谢工程策略及其在两种主要氨基酸生产微生物： 和 中的应用。
