Laboratory of OSG Veterinary Science for Global Disease Management, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Laboratory of Theriogenology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
J Vet Med Sci. 2024 Dec 1;86(12):1289-1293. doi: 10.1292/jvms.24-0298. Epub 2024 Nov 4.
A 4-year 9-month-old Holstein-Friesian dairy cow presented with anorexia. On physical examination, swelling of superficial lymph nodes, pelvic masses, and prolonged urination posture after urinating a small amount were noted. Hematological examination revealed no lymphocytosis. The bovine leukemia virus proviral load was relatively high. At necropsy, enlarged lymph nodes, a large mass in the pelvic cavity, and mass lesions in several organs were observed. Histopathological examination revealed the proliferation of neoplastic lymphocytes, which were immune-positive for CD79α and negative for CD3. B-cell clonality test indicated the presence of monoclonality in the urine, masses, and lymph nodes and minor clonality in the peripheral blood. These findings led to a diagnosis of EBL with minor clonality of B-cell in the peripheral blood.
一头 4 岁 9 个月大的荷斯坦弗里生奶牛出现食欲不振。在体格检查中，注意到浅表淋巴结肿大、盆腔肿块和排尿后长时间保持排尿姿势但仅排出少量尿液。血液检查未发现淋巴细胞增多。牛白血病病毒前病毒载量相对较高。在剖检时，观察到淋巴结肿大、盆腔内有一个大肿块，以及几个器官的肿块病变。组织病理学检查显示肿瘤性淋巴细胞增生，CD79α 免疫阳性而 CD3 阴性。B 细胞克隆性检测表明尿液、肿块和淋巴结存在单克隆性，外周血存在少量克隆性。这些发现提示存在 EBL，外周血中有 B 细胞的少量克隆性。
