Shekhovtsov Sergei V, Katokhin Alexei V, Kolchanov Nikolai A, Mordvinov Viatcheslav A
The Institute Of Cytology and Genetics, Novosibirsk, Russian Federation.
Parasitol Int. 2010 Mar;59(1):100-3. doi: 10.1016/j.parint.2009.10.012. Epub 2009 Nov 10.
The complete mitochondrial genomes of the parasitic trematodes Opisthorchis felineus and Clonorchis sinensis (family Opisthorchiidae) were fully sequenced in order to develop markers for DNA diagnostics of the liver flukes infection, molecular ecology, population and phylogenetic studies. The complete sequences of mitochondrial genomes of these species comprise 14,277 and 13,875bp, respectively, and are thus the shortest trematode mitochondrial genomes sequenced to date. The gene content and arrangement are identical to that of Fasciola hepatica. ATG and GTG are used as the start-codons and TAG and TAA are used as the stop-codons. The stop-codon TAG of the C. sinensis nad1 gene overlap by 1nt with the downstream tRNA-Asn gene. Alternative structures for the Ser(UCN) tRNAs were found for both species. The noncoding control regions are separated into two parts by the tRNA-Gly gene and contain neither tandem repeats, which are characteristic for trematode control regions, nor secondary structures. In conclusion, the complete mitochondrial DNA sequences of O. felineus and C. sinensis will serve as a resource for comparative mitochondrial genomics and systematic studies of parasitic trematodes.
为开发用于肝吸虫感染的DNA诊断、分子生态学、种群和系统发育研究的标记，对寄生吸虫猫后睾吸虫和华支睾吸虫（后睾科）的完整线粒体基因组进行了全序列测定。这两个物种线粒体基因组的完整序列分别为14,277bp和13,875bp，是迄今为止测序的最短的吸虫线粒体基因组。基因内容和排列与肝片吸虫相同。ATG和GTG用作起始密码子，TAG和TAA用作终止密码子。华支睾吸虫nad1基因的终止密码子TAG与下游的tRNA-Asn基因重叠1个核苷酸。在这两个物种中均发现了Ser(UCN) tRNA的替代结构。非编码控制区被tRNA-Gly基因分为两部分，既不包含吸虫控制区特有的串联重复序列，也不包含二级结构。总之，猫后睾吸虫和华支睾吸虫的完整线粒体DNA序列将作为寄生吸虫比较线粒体基因组学和系统研究的资源。
