Department of Chemistry, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand.
Environ Pollut. 2010 Mar;158(3):730-6. doi: 10.1016/j.envpol.2009.10.015. Epub 2009 Nov 12.
Agricultural wastes are a source of steroid estrogens and, if present, conjugated estrogens may add to the estrogen load released to soil and aquatic environments. Dairy shed effluent samples were collected from 18 farms for analysis of steroid estrogens by GC-MS, conjugated estrogens by LC-MS-MS, and estrogenic activity by E-screen in vitro bioassay. 17alpha-estradiol was found at highest concentrations (median 730 ng l(-1)), followed by estrone (100 ng l(-1)) and 17beta-estradiol (24 ng l(-1)). Conjugated estrogens (estrone-3-sulfate, 17alpha-estradiol-3-sulfate and 17beta-estradiol-3,17-disulfate) were measured in most samples (12-320 ng l(-1)). Median estrogenic activity was 46 ng l(-1) 17beta-estradiol equivalents. Conjugated estrogens contributed up to 22% of the total estrogen load from dairy farming, demonstrating their significance. Steroid estrogens dominated overall estrogenic activity measured in the samples. Significantly, 17alpha-estradiol contributed 25% of overall activity, despite potency 2% that of 17beta-estradiol, highlighting the importance in environmental risk assessments of this previously neglected compound.
农业废弃物是甾体雌激素的一个来源，如果存在，结合雌激素可能会增加释放到土壤和水生环境中的雌激素负荷。从 18 个农场采集了奶牛棚污水样本，通过 GC-MS 分析甾体雌激素、LC-MS-MS 分析结合雌激素、E-screen 体外生物测定分析雌激素活性。17α-雌二醇的浓度最高（中位数为 730ng/L），其次是雌酮（100ng/L）和 17β-雌二醇（24ng/L）。大多数样本中都测量到了结合雌激素（雌酮-3-硫酸盐、17α-雌二醇-3-硫酸盐和 17β-雌二醇-3,17-二硫酸盐）（12-320ng/L）。雌二醇当量的中位数雌激素活性为 46ng/L。结合雌激素占奶牛养殖总雌激素负荷的 22%，表明其重要性。甾体雌激素在样品中测量的整体雌激素活性中占主导地位。值得注意的是，尽管 17α-雌二醇的效价仅为 17β-雌二醇的 2%，但其对总活性的贡献为 25%，这突出表明在环境风险评估中，这种以前被忽视的化合物的重要性。
