Department of Life Science and Engineering, Harbin Institute of echnology, Harbin, 150001, PR China.
J Microbiol. 2011 Feb;49(1):24-8. doi: 10.1007/s12275-011-0197-z. Epub 2011 Mar 3.
A moderately halophilic bacterial strain 15-13(T), which was isolated from soda meadow saline soil in Daqing City, Heilongjiang Province, China, was subjected to a polyphasic taxonomic study. The cells of strain 15-13 were found to be Gram-negative, rod-shaped, and motile. The required growth conditions for strain 15-13(T) were: 1-23% NaCl (optimum, 7%), 10-50°C (optimum, 35°C), and pH 7.0-11.0 (optimum, pH 9.5). The predominant cellular fatty acids were C(18:1) ω7c (60.48%) and C(16:0) (13.96%). The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 15-13(T) clustered within a branch comprising species of the genus Halomonas. The closest phylogenetic neighbor of strain 15-13(T) was Halomonas pantelleriensis DSM 9661(T) (98.9% 16S rRNA gene sequence similarity). The level of DNA-DNA relatedness between the novel isolated strain and H pantelleriensis DSM 9661(T) was 33.8%. On the basis of the phenotypic and phylogenetic data, strain 15-13(T) represents a novel species of the genus Halomonas, for which the name Halomonas alkalitolerans sp. nov. is proposed. The type strain for this novel species is 15-13(T) (=CGMCC 1.9129(T) =NBRC 106539(T)).
一株中度嗜盐的细菌 15-13(T)，它从中国黑龙江省大庆市苏打草地盐土中分离出来，经过多相分类学研究。发现 15-13(T)菌株的细胞呈革兰氏阴性、杆状和运动性。菌株 15-13(T)生长的需要条件为：1-23%的 NaCl（最佳为 7%）、10-50°C（最佳为 35°C）和 pH7.0-11.0（最佳为 pH9.5）。主要的细胞脂肪酸是 C(18:1)ω7c（60.48%）和 C(16:0)（13.96%）。DNA G+C 含量为 67.6mol%。基于 16S rRNA 基因序列比较的系统发育分析表明，菌株 15-13(T)聚在一个包含 Halomonas 属种的分支中。与菌株 15-13(T)最接近的系统发育邻居是 Halomonas pantelleriensis DSM 9661(T)（98.9%的 16S rRNA 基因序列相似性）。新分离菌株与 H pantelleriensis DSM 9661(T)之间的 DNA-DNA 相关性水平为 33.8%。根据表型和系统发育数据，菌株 15-13(T)代表了 Halomonas 属的一个新种，提议将其命名为 Halomonas alkalitolerans sp. nov.。该新种的模式株为 15-13(T)=CGMCC 1.9129(T)=NBRC 106539(T)。
