Hu Xing-Bin, Yue Qiao-Hong, Zhang Xian-Qing, Xu Xue-Qing, Wen Yin, Chen Yao-Zhen, Cheng Xiao-Dong, Yang Liu, Mu Shi-Jie
Department of Blood Transfusion, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
Virol J. 2009 Nov 17;6:199. doi: 10.1186/1743-422X-6-199.
Hepatitis B virus (HBV) is prevalent in China and screening of blood donors is mandatory. Up to now, ELISA has been universally used by the China blood bank. However, this strategy has sometimes failed due to the high frequency of nucleoside acid mutations. Understanding HBV evolution and strain diversity could help devise a better screening system for blood donors. However, this kind of information in China, especially in the northwest region, is lacking. In the present study, serological markers and the HBV DNA load of 11 samples from blood donor candidates from northwest China were determined. The HBV strains were most clustered into B and C genotypes and could not be clustered into similar types from reference sequences. Subsequent testing showed liver function impairment and increasing virus load in the positive donors. This HBV evolutionary data for China will allow for better ELISA and NAT screening efficiency in the blood bank of China, especially in the northwest region.
乙型肝炎病毒（HBV）在中国流行，对献血者进行筛查是强制性的。到目前为止，中国血库普遍使用酶联免疫吸附测定（ELISA）。然而，由于核苷酸突变频率高，这种策略有时会失败。了解HBV的进化和毒株多样性有助于设计出更好的献血者筛查系统。然而，在中国，尤其是西北地区，缺乏这类信息。在本研究中，测定了来自中国西北的11名献血候选者样本的血清学标志物和HBV DNA载量。HBV毒株大多聚为B型和C型，无法与参考序列聚为相似类型。随后的检测显示，阳性献血者存在肝功能损害且病毒载量增加。这些中国的HBV进化数据将提高中国血库，尤其是西北地区血库的ELISA和核酸扩增技术（NAT）筛查效率。
