妊娠期糖尿病患者的血浆apelin 水平和 apelin/APJ mRNA 表达。

Plasma apelin levels and apelin/APJ mRNA expression in patients with gestational diabetes mellitus.

机构信息

Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, Poland.

出版信息

Diabetes Res Clin Pract. 2010 Feb;87(2):176-83. doi: 10.1016/j.diabres.2009.10.018. Epub 2009 Nov 18.

DOI:10.1016/j.diabres.2009.10.018

PMID:19926159

Abstract

AIMS AND METHODS

Apelin is a novel adipokine identified as an endogenous ligand of the G protein-coupled receptor APJ. In this study we compared plasma apelin concentrations in 101 patients with gestational diabetes (GDM) and 101 women with normal glucose tolerance (NGT) between 24 and 32 weeks of gestation (Group 1), as well as in 20 women with GDM and 16 subjects with NGT at term (Group 2). Apelin and APJ mRNA expression in subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT) and placental tissue were also measured in Group 2, using quantitative real-time PCR.

RESULTS

There were no significant differences in plasma apelin levels between the women with GDM and NGT (Group 1: 1555.6 [1281.2-1804.2]pg/ml vs 1656.5 [1430.2-1852.1]pg/ml, Group 2: 1607.9 [1453.4-1768.7]pg/ml vs 1493.8 [1316.8-1956.7]pg/ml) nor in apelin and APJ mRNA expression in SAT, VAT and placental tissue. Apelin mRNA expression was approximately 10 fold higher in placental than in adipose tissue (p<0.0001). Apelin and APJ mRNA expression correlated significantly in SAT (R=0.45, p=0.03), VAT (R=0.69, p=0.003) and placental tissue (R=0.37, p=0.03).

CONCLUSIONS

No associations between circulating apelin or apelin/APJ mRNA expression and GDM or the indices of insulin resistance were noted in our study.

摘要

目的和方法

Apelin 是一种新型脂肪因子，被鉴定为 G 蛋白偶联受体 APJ 的内源性配体。在这项研究中，我们比较了 101 例妊娠期糖尿病（GDM）患者和 101 例糖耐量正常（NGT）孕妇在 24-32 孕周（第 1 组）以及 20 例 GDM 孕妇和 16 例 NGT 孕妇在足月（第 2 组）的血浆 Apelin 浓度。还使用定量实时 PCR 测量了第 2 组患者皮下脂肪组织（SAT）、内脏脂肪组织（VAT）和胎盘组织中的 Apelin 和 APJ mRNA 表达。

结果

GDM 患者和 NGT 患者的血浆 Apelin 水平没有差异（第 1 组：1555.6[1281.2-1804.2]pg/ml 与 1656.5[1430.2-1852.1]pg/ml，第 2 组：1607.9[1453.4-1768.7]pg/ml 与 1493.8[1316.8-1956.7]pg/ml），也没有在 SAT、VAT 和胎盘组织中的 Apelin 和 APJ mRNA 表达中观察到差异。Apelin mRNA 在胎盘组织中的表达比在脂肪组织中高约 10 倍（p<0.0001）。Apelin 和 APJ mRNA 在 SAT（R=0.45，p=0.03）、VAT（R=0.69，p=0.003）和胎盘组织（R=0.37，p=0.03）中存在显著相关性。