Federal University of Rio de Janeiro, Brazil.
Arch Oral Biol. 2010 Jan;55(1):21-8. doi: 10.1016/j.archoralbio.2009.10.005. Epub 2009 Nov 24.
To assess the prevalence of oral colonisation by bacterial respiratory pathogens in hospitalised patients.
Thirty patients undergoing myocardium revascularisation surgery were evaluated. At baseline (pre-operative phase), full-mouth clinical periodontal assessment was performed. Saliva and biofilm samples were obtained from subjects at baseline and at the post-operative phase, after orotracheal extubation. DNA was extracted from samples and species of Acinetobacter, Pseudomonas, Staphylococcus aureus and Dialister pneumosintes were detected by PCR or culture (for staphylococci isolates).
Most of the subjects were males, with history of hypertension and smoking. Thirteen were edentulous (ED) and 17 were dentate (DE), with moderate chronic periodontitis. The most prevalent bacteria in saliva were Staphylococcus spp. (85.7%), Pseudomonas spp. (83.8%), and Acinetobacter spp. (53.3%). There was a trend for D. pneumosintes to be more frequently detected in DE (43.7%) than ED (11.5%) patients. In plaque samples, DE with >14 teeth showed a higher prevalence of Pseudomonas spp. (100%) than individuals with < or =14 teeth (69.1%; p=0.048). Conversely, P. aeruginosa was more prevalent in subjects with fewer teeth (35.5%) than with >14 teeth (5.7%; p=0.037). All staphylococci isolates were coagulase-negative, and about 11% were positive for the mecA gene. These mecA-positive isolates showed a tendency to increase in all samples, whereas P. aeruginosa reduced after surgery. A strong correlation between the presence of Acinetobacter spp. and Pseudomonas spp. was observed (rho=0.886, p<0.05).
The oral cavity of hospitalised patients harbours high frequencies of bacterial respiratory pathogens, supporting its potential role as a reservoir for these species.
评估住院患者口腔中细菌呼吸道病原体的定植情况。
对 30 名接受心肌血运重建手术的患者进行评估。在基线（术前阶段）时，对患者进行全口临床牙周评估。在基线和术后阶段（气管拔管后），从患者中获得唾液和生物膜样本。从样本中提取 DNA，通过 PCR 或培养（针对葡萄球菌分离株）检测不动杆菌、铜绿假单胞菌、金黄色葡萄球菌和 Dialister pneumosintes 的种类。
大多数患者为男性，有高血压和吸烟史。13 名无牙（ED），17 名有牙（DE），患有中度慢性牙周炎。唾液中最常见的细菌是葡萄球菌属（85.7%）、假单胞菌属（83.8%）和不动杆菌属（53.3%）。DE 患者中 Dialister pneumosintes 的检出率（43.7%）高于 ED 患者（11.5%），有检出趋势。在菌斑样本中，有>14 颗牙齿的 DE 患者中假单胞菌属（100%）的检出率高于有≤14 颗牙齿的个体（69.1%；p=0.048）。相反，在牙齿较少的患者（35.5%）中，铜绿假单胞菌的检出率高于有>14 颗牙齿的患者（5.7%；p=0.037）。所有葡萄球菌分离株均为凝固酶阴性葡萄球菌，约 11%的分离株 mecA 基因阳性。这些 mecA 阳性分离株在所有样本中均呈增加趋势，而铜绿假单胞菌在手术后减少。不动杆菌属和假单胞菌属的存在呈强相关性（rho=0.886，p<0.05）。
住院患者的口腔中存在高频率的细菌呼吸道病原体，支持其作为这些物种的潜在储库的作用。
