Departamento de Biología Celular y Molecular, Universidad de Guadalajara, Carretera a Nogales Km 15.5, z.p. 45110, Las Agujas, Zapopan, Jalisco, Mexico.
Fish Shellfish Immunol. 2010 Feb;28(2):401-5. doi: 10.1016/j.fsi.2009.11.017. Epub 2009 Nov 24.
Endosulfan is a persistent organochlorine insecticide which is extremely toxic to fish. It is known to induce immunological alterations in juvenile Nile tilapia (Oreochromis niloticus) such as increases in phagocytic activity and reactive oxygen species production of spleen macrophages. The purpose of the present study was to demonstrate the effects of acute exposure to a sublethal concentration of endosulfan (7 ppb, 96 h) on parameters of the adaptive humoral immune response of the aforementioned aquatic organism. The effect of endosulfan on the capacity of immune cells to produce interleukin-2 like (IL-2L) factor and immunoglobulin M (IgM) in response to a challenge with (1/2) LD50 of the infectious bacteria Aeromonas hydrophila was evaluated. Experimental results indicate that short, sublethal, endosulfan exposure triggers a succession of events beginning with non-specific activation of macrophages followed by an exacerbated synthesis of the IL-2L factor by activated B cells. This leads to significantly increased secretion of IgM and could in turn facilitate autoantibody production and the development of autoimmune pathologies.
硫丹是一种持久性有机氯杀虫剂，对鱼类具有极高毒性。已知它会导致幼年尼罗罗非鱼（Oreochromis niloticus）发生免疫改变，如脾巨噬细胞的吞噬活性和活性氧物质的产生增加。本研究旨在证明亚致死浓度（7 ppb，96 h）的硫丹急性暴露对上述水生生物适应性体液免疫反应参数的影响。评估了硫丹对免疫细胞在受到（1/2）半数致死量的传染性细菌嗜水气单胞菌刺激时产生白细胞介素 2 样（IL-2L）因子和免疫球蛋白 M（IgM）的能力的影响。实验结果表明，短期、亚致死的硫丹暴露会引发一系列事件，首先是巨噬细胞的非特异性激活，随后是被激活的 B 细胞过度合成 IL-2L 因子。这会导致 IgM 的分泌显著增加，从而可能促进自身抗体的产生和自身免疫性疾病的发展。
