通过 ratiometric pre-rRNA 分析对水中存活细菌病原体的分子检测。
Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.
机构信息
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
出版信息
Appl Environ Microbiol. 2010 Feb;76(3):960-2. doi: 10.1128/AEM.01810-09. Epub 2009 Nov 30.
Abstract
Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.
摘要
比率型前 rRNA 分析（RPA）可检测到细菌细胞受到营养刺激后迅速补充 rRNA 前体的情况。与 PCR 检测 DNA 不同，RPA 可区分活细菌和失活细菌。它有望成为水质和其他环境样本中病原体的分子活力检测方法。
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