Adenot P G, Szöllösi M S, Geze M, Renard J P, Debey P
Unité en Développement Concerté INSERM U 310-INRA, Institut de Biologie Physico-Chimique, Paris, France.
Mol Reprod Dev. 1991 Jan;28(1):23-34. doi: 10.1002/mrd.1080280105.
Using video-enhanced fluorescence microscopy, we describe in live mouse zygotes the paternal chromatin changes undergone after fertilization. We focus on the sperm recondensation process and the formation of the paternal pronucleus, in relationship with the progression of maternal chromatin. Chromatin is labeled with the vital fluorophore Hoechst 33342. Our conditions of dye concentration and irradiation allow a continuous following of the dynamics of changes without major perturbation. We combine these observations with ultrastructural analysis performed by electron microscopy of the same eggs fixed at chosen stages. We show that the highly recondensed state corresponds to the appearance of the nuclear envelope and therefore the beginning of the pronuclear stage.
利用视频增强荧光显微镜，我们在活的小鼠受精卵中描述了受精后父本染色质发生的变化。我们关注精子再凝聚过程以及父本原核的形成，及其与母本染色质进程的关系。染色质用活性荧光染料Hoechst 33342标记。我们的染料浓度和照射条件能够在不造成重大干扰的情况下持续跟踪变化动态。我们将这些观察结果与通过电子显微镜对在选定阶段固定的相同卵子进行的超微结构分析相结合。我们发现，高度再凝聚状态对应于核膜的出现，因此是原核阶段的开始。
