Wilson Katherine E, Wilcke Jeff R, Crisman Mark V, Ward Daniel L, McKenzie Harold C, Scarratt W Kent
Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24060, USA.
Am J Vet Res. 2009 Dec;70(12):1545-50. doi: 10.2460/ajvr.70.12.1545.
Objective-To determine whether a limited sampling time method based on serum iohexol clearance (Cl(iohexol)) would yield estimates of glomerular filtration rate (GFR) in clinically normal horses similar to those for plasma creatinine clearance (Cl(creatinine)). Animals-10 clinically normal adult horses. Procedures-A bolus of iohexol (150 mg/kg) was administered IV, and serum samples were obtained 5, 20, 40, 60, 120, 240, and 360 minutes after injection. Urinary clearance of exogenous creatinine was measured during three 20-minute periods. The GFR determined by use of serum Cl(iohexol) and plasma Cl(creatinine) was compared with limits of agreement plots. Results-Values obtained for plasma Cl(creatinine) ranged from 1.68 to 2.69 mL/min/kg (mean, 2.11 mL/min/kg). Mean serum Cl(iohexol) was 2.38 mL/min/kg (range, 1.95 to 3.33 mL/min/kg). Limits of agreement plots indicated good agreement between the methods. Conclusions and Clinical Relevance-Use of serum Cl(iohexol) yielded estimates of GFR in clinically normal adult horses similar to those for plasma Cl(creatinine). This study was the first step in the evaluation of the use of serum Cl(iohexol) for estimating GFR in adult horses.
目的——确定基于血清碘海醇清除率（Cl(碘海醇)）的有限采样时间方法能否得出与血浆肌酐清除率（Cl(肌酐)）相似的临床正常马匹的肾小球滤过率（GFR）估计值。
动物——10匹临床正常的成年马。
步骤——静脉注射一剂碘海醇（150mg/kg），并在注射后5、20、40、60、120、240和360分钟采集血清样本。在三个20分钟时间段内测量外源性肌酐的尿清除率。使用血清Cl(碘海醇)和血浆Cl(肌酐)测定的GFR与一致性界限图进行比较。
结果——血浆Cl(肌酐)的值范围为1.68至2.69mL/（min·kg）（平均值为2.11mL/（min·kg））。血清Cl(碘海醇)的平均值为2.38mL/（min·kg）（范围为1.95至3.33mL/（min·kg））。一致性界限图表明两种方法之间具有良好的一致性。
结论及临床意义——使用血清Cl(碘海醇)得出的临床正常成年马的GFR估计值与血浆Cl(肌酐)的相似。本研究是评估血清Cl(碘海醇)用于估计成年马GFR的第一步。
