Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Int Immunopharmacol. 2010 Mar;10(3):290-7. doi: 10.1016/j.intimp.2009.11.013. Epub 2009 Dec 3.
Physalis angulata is an annual herb widely used in folk medicine. It is mainly used for treating rheumatoid arthritis (RA). Following bioactivity-guided isolation, a representative immunosuppressive compound, Physalin H was been identified from this herb medicine. The purpose of this work was to assess the immunosuppressive activity of Physalin H on T cells and to explore its potential mode of action. The results showed that Physalin H in a dose-dependent manner significantly inhibited the proliferation of T cells induced by concanavalin A (ConA) and by the mixed lymphocyte culture reaction (MLR). This inhibitive activity was mainly due to interfering DNA replication in G1 stages. In vivo experiments showed that, administration of Physalin H dose-dependently suppressed CD4(+) T cell mediated delayed-type hypersensitivity (DTH) reactions, and suppressed antigen-specific T-cell response in ovalbumin (OVA) immunized mice. Further study indicated that Physalin H could modulate Th1/Th2 cytokine balance and induce the production of immune regulation target Heme oxygenase (HO)-1 in T-cells in vitro. In this study, we demonstrated the immunosuppressive effect of Physalin H on T cells both in vitro and in vivo, and the immunosuppressive activity might be attributed to the suppression of T cell activation and proliferation, the modulation of Th1/Th2 cytokine balance and the induction of HO-1 in T cells.
番荔枝是一种一年生草本植物，广泛应用于民间医学。它主要用于治疗类风湿性关节炎（RA）。通过生物活性导向分离，从这种草药中鉴定出一种具有代表性的免疫抑制化合物——番荔枝 H。本工作旨在评估番荔枝 H 对 T 细胞的免疫抑制活性，并探讨其潜在的作用机制。结果表明，番荔枝 H 以剂量依赖的方式显著抑制刀豆蛋白 A（ConA）和混合淋巴细胞培养反应（MLR）诱导的 T 细胞增殖。这种抑制活性主要是由于干扰 G1 期的 DNA 复制。体内实验表明，番荔枝 H 给药剂量依赖性地抑制 CD4(+)T 细胞介导的迟发型超敏反应（DTH）反应，并抑制卵清蛋白（OVA）免疫小鼠的抗原特异性 T 细胞反应。进一步的研究表明，番荔枝 H 可以调节 Th1/Th2 细胞因子平衡，并在体外诱导 T 细胞产生免疫调节靶标血红素加氧酶（HO）-1。本研究证明了番荔枝 H 在体内外对 T 细胞的免疫抑制作用，其免疫抑制活性可能归因于抑制 T 细胞的活化和增殖、调节 Th1/Th2 细胞因子平衡以及诱导 T 细胞中 HO-1 的产生。
