光谱相位显微镜定量分析完整血红细胞中的血红蛋白浓度。
Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells.
机构信息
George R. Harrison Spectroscopy Laboratory, MIT, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
出版信息
Opt Lett. 2009 Dec 1;34(23):3668-70. doi: 10.1364/OL.34.003668.
Abstract
We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.
摘要
我们报告了一种使用样品诱导相移的光谱成像进行无标记定量特定分子的实用方法。配备各种波长光源的衍射相显微镜用于记录波长相关的相图像。我们首先对存在于细胞中的单一分子物种的纯溶液（如白蛋白和血红蛋白（Hb））进行色散测量。通过对分子特异性色散的这种预先校准，我们从单个人类红细胞中提取了 Hb 浓度。本研究的终点是对完整活细胞的生理状态进行非侵入性监测。
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本文引用的文献
1
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Opt Express. 2009 Jul 20;17(15):12285-92. doi: 10.1364/oe.17.012285.
2
Diffraction phase and fluorescence microscopy.衍射相衬显微镜和荧光显微镜。
Opt Express. 2006 Sep 4;14(18):8263-8. doi: 10.1364/oe.14.008263.
3
Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum.恶性疟原虫寄生的人类红细胞的折射率图谱和膜动力学
Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):13730-5. doi: 10.1073/pnas.0806100105. Epub 2008 Sep 4.
4
Optical imaging of cell mass and growth dynamics.细胞团块与生长动力学的光学成像
Am J Physiol Cell Physiol. 2008 Aug;295(2):C538-44. doi: 10.1152/ajpcell.00121.2008. Epub 2008 Jun 18.
5
Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium.利用双波长数字全息显微镜和灌注介质的染料增强色散进行细胞形态测量和折射率同步测量。
Opt Lett. 2008 Apr 1;33(7):744-6. doi: 10.1364/ol.33.000744.
6
Quantitative optical phase microscopy.定量光学相显微镜
Opt Lett. 1998 Jun 1;23(11):817-9. doi: 10.1364/ol.23.000817.
7
Digital holography for quantitative phase-contrast imaging.用于定量相衬成像的数字全息术。
Opt Lett. 1999 Mar 1;24(5):291-3. doi: 10.1364/ol.24.000291.
8
Interferometric phase-dispersion microscopy.
Opt Lett. 2000 Oct 15;25(20):1526-8. doi: 10.1364/ol.25.001526.
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