George R. Harrison Spectroscopy Laboratory, MIT, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Opt Lett. 2009 Dec 1;34(23):3668-70. doi: 10.1364/OL.34.003668.
We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.
我们报告了一种使用样品诱导相移的光谱成像进行无标记定量特定分子的实用方法。配备各种波长光源的衍射相显微镜用于记录波长相关的相图像。我们首先对存在于细胞中的单一分子物种的纯溶液（如白蛋白和血红蛋白（Hb））进行色散测量。通过对分子特异性色散的这种预先校准，我们从单个人类红细胞中提取了 Hb 浓度。本研究的终点是对完整活细胞的生理状态进行非侵入性监测。
