Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
PLoS One. 2009 Nov 23;4(11):e7948. doi: 10.1371/journal.pone.0007948.
Between 2003 and 2005, highly pathogenic avian influenza A (H5N1) viruses caused large scale outbreaks in poultry in the Ho Chi Minh City area in Vietnam. We studied the prevalence of antibodies against H5N1 in poultry workers and cullers who were active in the program in Ho Chi Minh City in 2004 and 2005.
METHODOLOGY/PRINCIPAL FINDINGS: Single sera from 500 poultry workers and poultry cullers exposed to infected birds were tested for antibodies to avian influenza H5N1, using microneutralization assays and hemagglutination inhibition assay with horse blood. All sera tested negative using microneutralization tests. Three samples showed a 1ratio80 titer in the hemagglutination inhibition assay.
CONCLUSIONS/SIGNIFICANCE: This study provides additional support for the low transmissibility of clade 1 H5N1 to humans, but limited transmission to highly exposed persons cannot be excluded given the presence of low antibody titers in some individuals.
2003 年至 2005 年间，高致病性禽流感 A（H5N1）病毒在越南胡志明市地区的家禽中大规模爆发。我们研究了 2004 年和 2005 年在胡志明市从事该项目的家禽工人和扑杀者中针对 H5N1 的抗体流行情况。
方法/主要发现：使用微量中和试验和马血血凝抑制试验，对 500 名接触感染鸟类的家禽工人和扑杀者的单份血清进行了抗禽流感 H5N1 的抗体检测。所有检测血清均采用微量中和试验检测呈阴性。三份样本在血凝抑制试验中显示 1：80 的效价。
结论/意义：本研究为 clade 1 H5N1 对人类的低传染性提供了更多支持，但鉴于一些个体中存在低抗体滴度，不能排除对高度暴露者的有限传播。
