图拉汉坦病毒 NSs 蛋白在感染和转染细胞中积聚在核周区。

Tula hantavirus NSs protein accumulates in the perinuclear area in infected and transfected cells.

机构信息

Infection Biology Research Program, Department of Virology, Haartman Institute, University of Helsinki, PO Box 21, Haartmaninkatu 3, 00014 Helsinki, Finland.

出版信息

Arch Virol. 2010;155(1):117-21. doi: 10.1007/s00705-009-0546-y. Epub 2009 Dec 3.

DOI:10.1007/s00705-009-0546-y

PMID:19956987

Abstract

The small RNA segment of some hantaviruses (family Bunyaviridae) encodes two proteins: the nucleocapsid protein and, in an overlapping reading frame, a non-structural (NSs) protein. The hantavirus NSs protein, like those of orthobunya- and phleboviruses, counteracts host innate immunity. Here, for the first time, the NSs protein of a hantavirus (Tula virus) has been observed in infected cells and shown to localize in the perinuclear area. Transiently expressed NSs protein showed similar localization, although the kinetics was slightly different, suggesting that to reach its proper location in the infected cell, the NSs protein does not have to cooperate with other viral proteins.

摘要

某些汉坦病毒（布尼亚病毒科）的小 RNA 片段编码两种蛋白：核衣壳蛋白和在重叠阅读框中的非结构（NSs）蛋白。汉坦病毒的 NSs 蛋白与正粘病毒和黄病毒的 NSs 蛋白一样，可拮抗宿主固有免疫。在这里，汉坦病毒（图拉病毒）的 NSs 蛋白首次在受感染的细胞中被观察到，并定位于核周区。瞬时表达的 NSs 蛋白显示出类似的定位，尽管动力学略有不同，这表明为了到达受感染细胞中的适当位置，NSs 蛋白不必与其他病毒蛋白合作。