Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland.
PLoS One. 2012;7(4):e34307. doi: 10.1371/journal.pone.0034307. Epub 2012 Apr 10.
Hantaviruses (Bunyaviridae) are negative-strand RNA viruses with a tripartite genome. The small (S) segment encodes the nucleocapsid protein and, in some hantaviruses, also the nonstructural protein (NSs). The aim of this study was to find potential cellular partners for the hantaviral NSs protein. Toward this aim, yeast two-hybrid (Y2H) screening of mouse cDNA library was performed followed by a search for potential NSs protein counterparts via analyzing a cellular interactome. The resulting interaction network was shown to form logical, clustered structures. Furthermore, several potential binding partners for the NSs protein, for instance ACBD3, were identified and, to prove the principle, interaction between NSs and ACBD3 proteins was demonstrated biochemically.
汉坦病毒（布尼亚病毒科）是具有三分体基因组的负链 RNA 病毒。小（S）片段编码核衣壳蛋白，在一些汉坦病毒中，还编码非结构蛋白（NSs）。本研究旨在寻找汉坦病毒 NSs 蛋白的潜在细胞伴侣。为此，进行了小鼠 cDNA 文库的酵母双杂交（Y2H）筛选，然后通过分析细胞相互作用组寻找潜在的 NSs 蛋白对应物。结果表明，所得的相互作用网络形成了逻辑上的聚类结构。此外，还鉴定出了几个 NSs 蛋白的潜在结合伴侣，例如 ACBD3，并且为了证明原理，还通过生化方法证明了 NSs 和 ACBD3 蛋白之间的相互作用。
