Gynaecology and Obstetrics Department, Peking University Third Hospital, Beijing, People's Republic of China.
Pathol Oncol Res. 2010 Sep;16(3):353-60. doi: 10.1007/s12253-009-9225-2. Epub 2009 Dec 3.
S100 proteins, a family of Ca(2+)-binding proteins, have been linked to several human diseases in recent years. Deregulated expression of S100 proteins, including S100A9 and its partner S100A8, was reported to be associated with neoplastic disorders. In our previous study using serial analysis of gene expression, we identified decreased expressions of S100A9 and S100A8 in human cervical squamous cell carcinoma. To investigate the functions of S100A8 and S100A9 in cervical cancer, we purified recombinant S100A8 and S100A9 proteins and treated CaSki human cervical cancer cells with these proteins. We found that S100A8/A9 induced apoptosis and inhibited migration of CaSki cells; S100A8/A9 also reduced the expression of matrix metalloproteinase (MMP)-2 in CaSki cells. In summary, this study suggests that S100A8 and S100A9 have inhibitory effects on the proliferation of CaSki carcinoma cells by inducing cell apoptosis and on the invasiveness of CaSki cells.
S100 蛋白是一类 Ca(2+)-结合蛋白，近年来与多种人类疾病有关。据报道，S100 蛋白的表达失调，包括 S100A9 及其伴侣 S100A8，与肿瘤性疾病有关。在我们之前使用基因表达序列分析的研究中，我们发现人宫颈鳞状细胞癌细胞中 S100A9 和 S100A8 的表达降低。为了研究 S100A8 和 S100A9 在宫颈癌中的功能，我们纯化了重组 S100A8 和 S100A9 蛋白，并将这些蛋白处理 CaSki 人宫颈癌细胞。我们发现 S100A8/A9 诱导 CaSki 细胞凋亡并抑制迁移；S100A8/A9 还降低了 CaSki 细胞中基质金属蛋白酶（MMP）-2 的表达。总之，这项研究表明，S100A8 和 S100A9 通过诱导细胞凋亡对 CaSki 癌细胞的增殖和 CaSki 细胞的侵袭具有抑制作用。
