Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.
Department of Chemical and Biological Engineering, Hanbat National University, Daejeon 34158, Korea.
Int J Mol Sci. 2021 Sep 26;22(19):10382. doi: 10.3390/ijms221910382.
The precise synthesis of fine-sized nanoparticles is critical for realizing the advantages of nanoparticles for various applications. We developed a technique for preparing finely controllable sizes of gold nanoparticles (Au NPs) on a silica template, using the seed-mediated growth and interval dropping methods. These Au NPs, embedded on silica nanospheres (SiO@Au NPs), possess peroxidase-like activity as nanozymes and have several advantages over other nanoparticle-based nanozymes. We confirmed their peroxidase activity; in addition, factors affecting the activity were investigated by varying the reaction conditions, such as concentrations of tetramethyl benzidine and HO, pH, particle amount, reaction time, and termination time. We found that SiO@Au NPs are highly stable under long-term storage and reusable for five cycles. Our study, therefore, provides a novel method for controlling the properties of nanoparticles and for developing nanoparticle-based nanozymes.
精细尺寸纳米粒子的精确合成对于实现纳米粒子在各种应用中的优势至关重要。我们开发了一种在二氧化硅模板上制备精细可控尺寸金纳米粒子（Au NPs）的技术，使用种子介导生长和间隔滴加方法。这些嵌入在二氧化硅纳米球（SiO@Au NPs）上的 Au NPs 作为纳米酶具有类过氧化物酶活性，并且比其他基于纳米粒子的纳米酶具有多个优势。我们证实了它们的过氧化物酶活性；此外，通过改变反应条件，例如四甲基联苯胺和 HO 的浓度、pH 值、颗粒量、反应时间和终止时间，研究了影响活性的因素。我们发现，SiO@Au NPs 在长期储存下非常稳定，并且可以重复使用五轮。因此，我们的研究为控制纳米粒子的性质和开发基于纳米粒子的纳米酶提供了一种新方法。
