人源纳米颗粒形成的生物膜的特性。
Characterization of biofilm formed by human-derived nanoparticles.
机构信息
Department of Physiology & Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, MN 55905, USA.
出版信息
Nanomedicine (Lond). 2009 Dec;4(8):931-41. doi: 10.2217/nnm.09.72.
AIM
Microbial biofilm matrix contains polysaccharides and proteins and can require extracellular nucleic acids for initial formation. Experiments were designed to identify infectious pathogens in human aneurysms and to characterize biofilm formed by calcified human arterial-derived nanoparticles.
MATERIALS & METHOD: A total of 26 different microbial pathogens were isolated from 48 inflammatory aneurysms. Consistent amounts (0.49 McFarland units) of nanoparticles derived from similar tissue were seeded into 24-well plates and cultured for 21 days in the absence (control) or presence of RNase, tetracycline or gentamicin.
RESULTS
Control biofilm developed within 14 days, as detected by concanavalin A and BacLight Green staining. The formation of biofilm in wells treated with RNase was not different from the control; however, gentamicin partially inhibited and tetracycline completely inhibited biofilm formation. Therefore, nanoparticle biofilm retains some characteristics of conventional bacterial biofilm and requires protein-calcium interactions, although extracellular RNA is not required.
CONCLUSION
This model system may also allow study of nanosized vesicles derived from donor tissue, including any microbes present, and could provide a useful tool for in vitro investigation of nanoparticle biofilm formation.
目的
微生物生物膜基质含有多糖和蛋白质，并且初始形成可能需要细胞外核酸。设计实验以鉴定人动脉瘤中的感染性病原体，并对钙化的人动脉衍生纳米颗粒形成的生物膜进行特征描述。
材料与方法
从 48 个炎症性动脉瘤中分离出 26 种不同的微生物病原体。从类似组织中提取出等量（0.49 McFarland 单位）的纳米颗粒，播种到 24 孔板中，在无（对照）或存在 RNA 酶、四环素或庆大霉素的情况下培养 21 天。
结果
通过 Concanavalin A 和 BacLight Green 染色检测到，对照生物膜在 14 天内形成。用 RNA 酶处理的孔中生物膜的形成与对照没有区别；然而，庆大霉素部分抑制，四环素完全抑制生物膜的形成。因此，纳米颗粒生物膜保留了传统细菌生物膜的一些特征，尽管不需要细胞外 RNA，但需要蛋白-钙相互作用。
结论
该模型系统还可以研究供体组织来源的纳米尺寸囊泡，包括任何存在的微生物，并且可以为体外研究纳米颗粒生物膜形成提供有用的工具。
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