Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, India.
Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Tamilnadu, India.
In Vitro Cell Dev Biol Anim. 2023 Jun;59(6):467-478. doi: 10.1007/s11626-023-00782-2. Epub 2023 Jul 19.
Developing blood vessels from the existing vasculature is vital for the growth of the organism, as well as for systematic wound healing and the repair process. In this study, we investigated the role of angiogenesis during the regeneration process in the earthworm, Eudrilus eugeniae, animal model. Briefly, the morphological examination of blood vessels in juvenile and mature worms is documented, along with the development of new blood vessels in regenerating blastema. However, in vivo and in vitro experiments with juvenile worms revealed that geraniol retards blastemal regeneration growth with undeveloped blood vessels, as compared to the control. The results of qRT-PCR, western blotting, and immunohistochemistry confirmed a reduced expression of VEGFR2 and WNT5A in the day 3 regenerating blastema of geraniol-treated worms, as compared to the control. We conclude that geraniol acts as a potent natural inhibitor of angiogenesis, thereby retarding the regeneration process in earthworms. In addition, for studying angiogenesis and screening effective angiogenesis inhibitors as drug candidates, the earthworm is an ambient animal model system.
从现有脉管系统中开发血管对于生物体的生长以及系统性伤口愈合和修复过程至关重要。在这项研究中，我们调查了在蚯蚓（Eudrilus eugeniae）动物模型的再生过程中血管生成的作用。简要地说，记录了幼年和成年蚯蚓中血管的形态学检查，以及再生芽中新生血管的发育情况。然而，对幼年蚯蚓进行的体内和体外实验表明，与对照相比，香叶醇会延迟芽生再生生长，同时伴有血管发育不良。qRT-PCR、western blot 和免疫组织化学的结果证实，与对照相比，香叶醇处理的蚯蚓第 3 天再生芽中的 VEGFR2 和 WNT5A 表达减少。我们得出结论，香叶醇是一种有效的天然血管生成抑制剂，从而延迟了蚯蚓的再生过程。此外，对于研究血管生成和筛选有效的血管生成抑制剂作为药物候选物，蚯蚓是一种理想的动物模型系统。
