来自BALB/c小鼠的B型嗜异性白血病病毒的多态性：p30抗原与N型和B型嗜异性的关联。
Polymorphism of B-tropic leukemia viruses from BALB/c mice: association of a p30 antigen with N- versus B-tropism.
作者信息
Tress E, O'Donnell P V, Famulari N, Ellis R W, Fleissner E
出版信息
J Virol. 1979 Oct;32(1):350-5. doi: 10.1128/JVI.32.1.350-355.1979.
Abstract
Comparison of a number of murine leukemia virus clones by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed extensive protein polymorphism among B-tropic, but not N-tropic, isolates from BALB/c mice, particularly in migration of p30 proteins. A type-specific radioimmunoassay for p30 was developed which uniformly discriminated all B-tropic viruses from N-tropic viruses of BALB/c origin. N- and B-tropic viruses of C57BL/6 and AKR Fv-1b/b origin could also be distinguished by this assay.
摘要
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对多个鼠白血病病毒克隆进行比较，结果显示，从BALB/c小鼠中分离出的嗜B性而非嗜N性毒株之间存在广泛的蛋白质多态性，尤其是p30蛋白的迁移情况。开发了一种针对p30的型特异性放射免疫测定法，该方法能一致地区分所有来自BALB/c的嗜B性病毒和嗜N性病毒。该测定法也可区分C57BL/6和AKR Fv-1b/b来源的嗜N性和嗜B性病毒。
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