Lee Shin Ae, Kim Jeong Myeong, Ahn Jae-Hyung, Joa Jae-Ho, Kim Soo-Jin, Sang Mee-Kyung, Song Jaekyeong, Kwon Soon-Wo, Weon Hang-Yeon
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju, 55365, Republic of Korea.
Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal Science, RDA, Jeju, 63240, Republic of Korea.
J Microbiol. 2016 Nov;54(11):709-712. doi: 10.1007/s12275-016-6451-7. Epub 2016 Oct 29.
Strain 63MJ-2 was isolated from the feces of broad-winged katydid (Pseudorhynchus japonicus) collected in Korea. The 16S rRNA gene sequence of this strain showed the highest sequence similarity with that of Siphonobacter aquaeclarae P2 (96.1%) and had low similarities (below 86.3%) with those of other members of family 'Flexibacteraceae'. The strain 63MJ-2 is a strictly aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium. The strain grew at 4-35°C (optimum, 25-30°C), pH of 5.0-9.0 (optimum, 6.0-7.0), and 0-2.0% (optimum, 1.0-2.0) (w/v) NaCl. The DNA G+C content of strain 63MJ-2 was 43.5 mol%. The major fatty acids were C ω5c (42.5%), iso-C 3-OH (18.7%), and summed feature 3 (iso-C 2-OH and/or C ω7c, 18.0%). The major menaquinone was MK-7 and polar lipids were phosphatidylethanolamine, six unknown aminolipids, and five unknown lipids. Based on the evidence from our polyphasic taxonomic study, we conclude that strain 63MJ-2 should be classified as a novel species of the genus Siphonobacter, and propose the name Siphonobacter intestinalis sp. nov. The type strain is 63MJ-2 (=KACC 18663 =NBRC 111883).
菌株63MJ - 2是从韩国采集的阔翅螽斯（Pseudorhynchus japonicus）粪便中分离得到的。该菌株的16S rRNA基因序列与清澈虹吸杆菌P2（Siphonobacter aquaeclarae P2）的序列相似性最高（96.1%），与“弯曲杆菌科”（Flexibacteraceae）其他成员的序列相似性较低（低于86.3%）。菌株63MJ - 2是严格需氧、革兰氏染色阴性、不运动的杆状细菌。该菌株在4 - 35°C（最适温度为25 - 30°C）、pH值5.0 - 9.0（最适pH值为6.0 - 7.0）和0 - 2.0%（最适浓度为1.0 - 2.0%）（w/v）NaCl条件下生长。菌株63MJ - 2的DNA G + C含量为43.5 mol%。主要脂肪酸为C ω5c（42.5%）、异-C 3-OH（18.7%）和总和特征3（异-C 2-OH和/或C ω7c，18.0%）。主要甲基萘醌为MK - 7，极性脂为磷脂酰乙醇胺、六种未知氨基脂和五种未知脂。基于我们多相分类研究的证据，我们得出结论，菌株63MJ - 2应被归类为虹吸杆菌属的一个新物种，并提议命名为肠道虹吸杆菌（Siphonobacter intestinalis sp. nov.）。模式菌株为63MJ - 2（=KACC 18663 =NBRC 111883）。
