Baik Keun Sik, Kim Mi Sun, Park Seong Chan, Lee Dong Wan, Lee Soon Dong, Ka Jong-Ok, Choi Sang Ki, Seong Chi Nam
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2007 Dec;57(Pt 12):2870-2873. doi: 10.1099/ijs.0.65302-0.
A Gram-negative, yellow-pigmented bacterium capable of gliding motility, designated strain WPCB118(T), was isolated from fresh water collected from the Woopo wetland (Republic of Korea). Cells were rod-shaped and sometimes filamentous. The major fatty acids were iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c (45.6 %), C(16 : 1)omega5c (18.5 %), iso-C(15 : 0) (9.5 %) and C(16 : 0) (8.8 %). The predominant menaquinone and polar lipid were MK-7 and phosphatidylethanolamine, respectively. The DNA G+C content was 53.3 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB118(T) had an evolutionary lineage within the radiation encompassing the members of the family 'Flexibacteraceae', its closest neighbour being Spirosoma linguale LMG 10896(T) (93.7 % gene sequence similarity). Data from this polyphasic study indicated that strain WPCB118(T) could not be assigned to any recognized species. Strain WPCB118(T) represents a novel species of the genus Spirosoma, for which the name Spirosoma rigui sp. nov. is proposed. The type strain is WPCB118(T) (=KCTC 12531(T)=NBRC 101117(T)).
从韩国沃浦湿地采集的淡水中分离出一株革兰氏阴性、产黄色色素、具有滑行运动能力的细菌，命名为菌株WPCB118(T)。细胞呈杆状，有时呈丝状。主要脂肪酸为异-C(15 : 0) 2-OH和/或C(16 : 1)ω7c（45.6%）、C(16 : 1)ω5c（18.5%）、异-C(15 : 0)（9.5%）和C(16 : 0)（8.8%）。主要甲基萘醌和极性脂质分别为MK-7和磷脂酰乙醇胺。DNA G+C含量为53.3 mol%。基于16S rRNA基因序列构建的系统发育树表明，菌株WPCB118(T)在包含“弯曲杆菌科”成员的辐射范围内具有一个进化谱系，其最接近的邻居是舌螺旋体LMG 10896(T)（基因序列相似性为93.7%）。这项多相研究的数据表明，菌株WPCB118(T)不能归属于任何已认可的物种。菌株WPCB118(T)代表螺旋体属的一个新物种，为此提出新名称rigui螺旋体（Spirosoma rigui sp. nov.）。模式菌株为WPCB118(T)（=KCTC 12531(T)=NBRC 101117(T)）。
