Yen Ching-Yu, Lu Min-Chi, Tzeng Ching-Cherng, Huang Jia-Yan, Chang Hsueh-Wei, Chen Ruey-Shyang, Liu Shyun-Yeu, Liu Shih-Tung, Shieh Biehuoy, Li Ching
Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Tainan 710, Taiwan.
J Biomed Biotechnol. 2009;2009:904589. doi: 10.1155/2009/904589.
Epstein-Barr virus is known to cause nasopharyngeal carcinoma. Although oral cavity is located close to the nasal pharynx, the pathogenetic role of Epstein-Barr virus (EBV) in oral cancers is unclear. This molecular epidemiology study uses EBV genomic microarray (EBV-chip) to simultaneously detect the prevalent rate and viral gene expression patterns in 57 oral squamous cell carcinoma biopsies (OSCC) collected from patients in Taiwan. The majority of the specimens (82.5%) were EBV-positive that probably expressed coincidently the genes for EBNAs, LMP2A and 2B, and certain structural proteins. Importantly, the genes fabricated at the spots 61 (BBRF1, BBRF2, and BBRF3) and 68 (BDLF4 and BDRF1) on EBV-chip were actively expressed in a significantly greater number of OSCC exhibiting exophytic morphology or ulceration than those tissues with deep invasive lesions (P = .0265 and .0141, resp.). The results may thus provide the lead information for understanding the role of EBV in oral cancer pathogenesis.
已知爱泼斯坦-巴尔病毒可引发鼻咽癌。尽管口腔与鼻咽部位置相邻，但爱泼斯坦-巴尔病毒（EBV）在口腔癌发病机制中的作用尚不清楚。这项分子流行病学研究采用EBV基因组微阵列（EBV芯片），同时检测从台湾患者收集的57例口腔鳞状细胞癌活检样本（OSCC）中的流行率和病毒基因表达模式。大多数标本（82.5%）为EBV阳性，可能同时表达EBNA、LMP2A和2B基因以及某些结构蛋白。重要的是，与那些有深层浸润性病变的组织相比，EBV芯片上第61个点（BBRF1、BBRF2和BBRF3）和第68个点（BDLF4和BDRF1）所构建的基因在显著更多的表现为外生性形态或溃疡的OSCC中活跃表达（分别为P = 0.0265和0.0141）。因此，这些结果可能为理解EBV在口腔癌发病机制中的作用提供先导信息。
