血管内皮生长因子在大鼠临界大小颅骨缺损中的有效应用。
Effectable application of vascular endothelial growth factor to critical sized rat calvaria defects.
作者信息
Yonamine Yutaka, Matsuyama Takashi, Sonomura Takahiro, Takeuchi Hironobu, Furuichi Yasushi, Uemura Masanori, Izumi Yuichi, Noguchi Kazuyuki
机构信息
Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Feb;109(2):225-31. doi: 10.1016/j.tripleo.2009.09.010.
OBJECTIVE
An early vascular response for angiogenesis is essential for the normal progression of bone defect healing. Vascular endothelial growth factor (VEGF) is a potent inducer of angiogenesis. The aim of this study was to evaluate the effects of a poly (L,D-lactic-co-glycolic acid) (PLGA) membrane with VEGF encapsulated into PLGA microspheres on bone regeneration at bone defects in rat calvaria.
STUDY DESIGN
Microspheres of PLGA incorporating VEGF(165) (VEGF microspheres) were prepared, and critical-size bone defects were created in rat calvaria. The VEGF microspheres, PLGA microspheres, or VEGF microspheres plus PLGA membrane were applied to the defects. Bone regeneration was evaluated using image analysis based on soft radiographic and histologic examination.
RESULTS
Mature thick bone regeneration was observed in selected sites at bone defects that had been applied with VEGF microspheres/PLGA membrane compared with those that had been applied with the other treatments.
CONCLUSION
A combination of VEGF microspheres and a PLGA membrane effectively enhances bone regeneration.
目的
血管生成的早期血管反应对于骨缺损愈合的正常进展至关重要。血管内皮生长因子（VEGF）是一种强大的血管生成诱导剂。本研究的目的是评估一种将VEGF包裹在聚（L，D-乳酸-乙醇酸共聚物）（PLGA）微球中的PLGA膜对大鼠颅骨骨缺损处骨再生的影响。
研究设计
制备了包含VEGF（165）的PLGA微球（VEGF微球），并在大鼠颅骨上制造了临界尺寸的骨缺损。将VEGF微球、PLGA微球或VEGF微球加PLGA膜应用于缺损处。基于软X线摄影和组织学检查，使用图像分析评估骨再生情况。
结果
与接受其他治疗的部位相比，在应用VEGF微球/PLGA膜的骨缺损选定部位观察到成熟的厚骨再生。
结论
VEGF微球和PLGA膜的组合有效地促进了骨再生。
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