Department of Toxicology, University of Wuerzburg, Wuerzburg, Germany.
Toxicol Lett. 2010 Mar 1;193(1):124-30. doi: 10.1016/j.toxlet.2009.12.016. Epub 2009 Dec 29.
Mobile phones are being used extensively throughout the world, with more than four billion accounts existing in 2009. This technology applies electromagnetic radiation in the microwave range. Health effects of this radiation have been subject of debate for a long time, both within the scientific community and within the general public. This study investigated the effect of mobile phone use on genomic instability of the human oral cavity's mucosa cells. 131 Individuals donated buccal mucosa cells extracted by slightly scraping the oral cavity with a cotton swab. Every participant filled out a questionnaire about mobile phone use including duration of weekly use, overall period of exposure and headset usage. 13 Individuals did not use mobile phones at all, 85 reported using the mobile phone for three hours per week or less, and 33 reported use of more than three hours per week. Additionally, information on age, gender, body weight, smoking status, medication and nutrition was retrieved. For staining of the cells a procedure using alpha-tubulin-antibody and chromomycin A(3) was applied. Micronuclei and other markers were evaluated in 1000 cells per individual at the microscope. A second scorer counted another 1000 cells, resulting in 2000 analyzed cells per individual. Mobile phone use did not lead to a significantly increased frequency of micronuclei.
手机在全球范围内被广泛使用，截至 2009 年，全球手机用户已超过 40 亿。这项技术应用的是微波范围内的电磁辐射。这种辐射对健康的影响长期以来一直是科学界和公众争论的话题。本研究调查了手机使用对人类口腔粘膜细胞基因组不稳定性的影响。131 名个体捐献了通过用棉签轻轻擦拭口腔提取的颊粘膜细胞。每位参与者都填写了一份关于手机使用的问卷，包括每周使用时长、总暴露时间和耳机使用情况。13 名个体完全不使用手机，85 名个体报告每周使用手机不超过 3 小时，33 名个体报告每周使用手机超过 3 小时。此外，还获取了年龄、性别、体重、吸烟状况、用药和营养方面的信息。对于细胞染色，采用了α-微管蛋白抗体和 Chromomycin A(3)的程序。在显微镜下，对每个人的 1000 个细胞进行微核和其他标记物的评估。第二位评分员对另外 1000 个细胞进行计数，每个个体分析了 2000 个细胞。手机使用并未导致微核的频率显著增加。
