Pandit Pranali, Singh Anita, Bafna A R, Kadam P V, Patil M J
Indian J Pharm Sci. 2008 Jul-Aug;70(4):440-4. doi: 10.4103/0250-474X.44590.
The ethanol extract of Curculigo orchioides was evaluated for antiasthmatic activity by using various in vitro and in vivo animal models. In vitro models like isolated goat tracheal chain preparation and isolated guinea pig ileum preparation were studied to know basic mechanism by which extract shows relaxant activity. The study showed that extract is effective against histamine-induced contraction. In isolated goat tracheal chain preparation and isolated guinea pig ileum preparation extract exhibits maximum relaxant effect (p< 0.01) against histamine at concentrations 100mug/ml and 25mug/ml respectively. Animal studies involved use of histamine induced bronchoconstriction in guinea pigs, egg albumin induced passive paw anaphylaxis in rats and haloperidol-induced catalepsy in mice. These studies showed significant (p< 0.01) protection at lower doses while further increase in the dose level showed reduced activity. Biochemical estimations in milk-induced total leukocytes count and milk-induced differential leukocyte count were also studied. In this study there was maximum increase in leucocytes and lymphocytes (99%) and maximum decrease in eosinophils up to 0% at dose 375mg/kg p.o. body weight was observed. The results of these studies indicated usefulness of ethanol extract of Curculigo orchioides in asthma.
通过使用各种体外和体内动物模型，对仙茅乙醇提取物的抗哮喘活性进行了评估。研究了诸如离体山羊气管链制备和离体豚鼠回肠制备等体外模型，以了解提取物显示松弛活性的基本机制。研究表明，该提取物对组胺诱导的收缩有效。在离体山羊气管链制备和离体豚鼠回肠制备中，提取物分别在浓度为100μg/ml和25μg/ml时对组胺表现出最大的松弛作用（p<0.01）。动物研究包括使用组胺诱导豚鼠支气管收缩、卵清蛋白诱导大鼠被动足过敏反应以及氟哌啶醇诱导小鼠僵住症。这些研究表明，在较低剂量时具有显著（p<0.01）的保护作用，而剂量水平进一步增加时活性降低。还研究了牛奶诱导的总白细胞计数和牛奶诱导的分类白细胞计数的生化评估。在本研究中，观察到在口服体重375mg/kg剂量时，白细胞和淋巴细胞增加最多（99%），嗜酸性粒细胞减少最多，降至0%。这些研究结果表明仙茅乙醇提取物在哮喘治疗中具有应用价值。
