Institute of Biomaterials and Biomedical Engineering, and Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.
J Clin Invest. 2010 Jan;120(1):60-70. doi: 10.1172/JCI41158.
Enabling stem cell-targeted therapies requires an understanding of how to create local microenvironments (niches) that stimulate endogenous stem cells or serve as a platform to receive and guide the integration of transplanted stem cells and their derivatives. In vivo, the stem cell niche is a complex and dynamic unit. Although components of the in vivo niche continue to be described for many stem cell systems, how these components interact to modulate stem cell fate is only beginning to be understood. Using the HSC niche as a model, we discuss here microscale engineering strategies capable of systematically examining and reconstructing individual niche components. Synthetic stem cell-niche engineering may form a new foundation for regenerative therapies.
实现干细胞靶向治疗需要了解如何构建局部微环境（龛），以刺激内源性干细胞或作为接收和指导移植干细胞及其衍生物整合的平台。在体内，干细胞龛是一个复杂而动态的单元。尽管许多干细胞系统的体内龛成分仍在不断被描述，但这些成分如何相互作用来调节干细胞命运才刚刚开始被理解。本文以造血干细胞龛为例，讨论了能够系统地检测和重建单个龛成分的微尺度工程策略。合成的干细胞-龛工程可能为再生疗法提供新的基础。
