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蛋白质溶液和组织中的磁共振水质子弛豫：T(1rho) 弥散特性分析。

Magnetic resonance water proton relaxation in protein solutions and tissue: T(1rho) dispersion characterization.

机构信息

Duke Cardiovascular Magnetic Resonance Center, Duke University Medical Center, Durham, North Carolina, United States of America.

出版信息

PLoS One. 2010 Jan 5;5(1):e8565. doi: 10.1371/journal.pone.0008565.

DOI:10.1371/journal.pone.0008565
PMID:20052404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2797329/
Abstract

BACKGROUND

Image contrast in clinical MRI is often determined by differences in tissue water proton relaxation behavior. However, many aspects of water proton relaxation in complex biological media, such as protein solutions and tissue are not well understood, perhaps due to the limited empirical data.

PRINCIPAL FINDINGS

Water proton T(1), T(2), and T(1rho) of protein solutions and tissue were measured systematically under multiple conditions. Crosslinking or aggregation of protein decreased T(2) and T(1rho), but did not change high-field T(1). T(1rho) dispersion profiles were similar for crosslinked protein solutions, myocardial tissue, and cartilage, and exhibited power law behavior with T(1rho)(0) values that closely approximated T(2). The T(1rho) dispersion of mobile protein solutions was flat above 5 kHz, but showed a steep curve below 5 kHz that was sensitive to changes in pH. The T(1rho) dispersion of crosslinked BSA and cartilage in DMSO solvent closely resembled that of water solvent above 5 kHz but showed decreased dispersion below 5 kHz.

CONCLUSIONS

Proton exchange is a minor pathway for tissue T(1) and T(1rho) relaxation above 5 kHz. Potential models for relaxation are discussed, however the same molecular mechanism appears to be responsible across 5 decades of frequencies from T(1rho) to T(1).

摘要

背景

临床 MRI 中的图像对比度通常由组织中水质子弛豫行为的差异决定。然而，复杂生物介质（如蛋白质溶液和组织）中水质子弛豫的许多方面尚未得到很好的理解，这可能是由于经验数据有限。

主要发现

在多种条件下，系统测量了蛋白质溶液和组织的水质子 T(1)、T(2)和 T(1rho)。蛋白质的交联或聚集降低了 T(2)和 T(1rho)，但没有改变高场 T(1)。交联蛋白溶液、心肌组织和软骨的 T(1rho)弥散谱相似，呈幂律行为，T(1rho)(0)值接近 T(2)。可移动蛋白质溶液的 T(1rho)弥散在 5 kHz 以上呈平坦状，但在 5 kHz 以下呈陡峭曲线，对 pH 值的变化敏感。DMSO 溶剂中交联 BSA 和软骨的 T(1rho)弥散在 5 kHz 以上与水溶剂非常相似，但在 5 kHz 以下弥散减少。

结论

质子交换是 5 kHz 以上组织 T(1)和 T(1rho)弛豫的次要途径。讨论了潜在的弛豫模型，但相同的分子机制似乎在从 T(1rho)到 T(1)的 5 个数量级频率范围内都起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/220fad570e54/pone.0008565.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/b170237c851e/pone.0008565.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/70190bdac175/pone.0008565.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/2618ca00e32a/pone.0008565.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/7d978777dd24/pone.0008565.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/15a61d23ad7f/pone.0008565.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/a166c35fd496/pone.0008565.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/220fad570e54/pone.0008565.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/b170237c851e/pone.0008565.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/1328ba318f61/pone.0008565.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/70190bdac175/pone.0008565.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/2618ca00e32a/pone.0008565.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/7d978777dd24/pone.0008565.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/15a61d23ad7f/pone.0008565.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/a166c35fd496/pone.0008565.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81c1/2797329/220fad570e54/pone.0008565.g008.jpg

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