Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA.
Microvasc Res. 2010 Mar;79(2):109-13. doi: 10.1016/j.mvr.2009.12.003. Epub 2010 Jan 4.
Accessibility to the bulbar conjunctival microvasculature provides a means to assess blood supply to the cerebral cortex and thus optimize therapeutic interventions designed to prevent or reduce the risk of cerebral vascular disease and stroke. The feasibility of a method for quantitative measurements of conjunctiva blood vessel diameter, blood velocity, and flow in the human eye is reported. The method is based on slit lamp biomicroscope digital imaging coupled with a space time image analysis technique. A sequence of conjunctiva microvasculature images was captured at a rate of 50 Hz. The images were analyzed to determine blood vessel diameter, velocity and flow. Blood vessel diameter measurements ranged between 8.7 and 24.3 microns, with a mean value of 15.5 microns. Blood flow rate ranged between 27.3 and 296.9 pl/s, with a mean value of 111.8 pl/s. The relationship between blood flow and vessel diameter was fit with a power law curve (R=0.87). The application of this technique for in vivo quantitative assessment of blood flow dynamics has potential to impact diagnosis and monitoring of various cardiovascular and blood disorders.
球结膜微脉管系统的可及性为评估大脑皮层的血液供应提供了一种手段，从而优化旨在预防或降低脑血管疾病和中风风险的治疗干预措施。本文报道了一种用于定量测量人眼结膜血管直径、血流速度和流量的方法。该方法基于裂隙灯生物显微镜数字成像技术和时空图像分析技术。以 50 Hz 的速率采集一系列球结膜微血管图像。对图像进行分析以确定血管直径、速度和流量。血管直径测量范围为 8.7 至 24.3 微米，平均值为 15.5 微米。血流速率范围为 27.3 至 296.9 pl/s，平均值为 111.8 pl/s。血流与血管直径之间的关系符合幂律曲线（R=0.87）。该技术在活体血流动力学定量评估中的应用具有影响各种心血管和血液疾病的诊断和监测的潜力。
