Drago J, Murphy M, Carroll S M, Harvey R P, Bartlett P F
Walter and Eliza Hall Institute of Medical Research, Royal Parade, Parkville, Victoria, Australia.
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2199-203. doi: 10.1073/pnas.88.6.2199.
Fibroblast growth factor stimulates proliferation and subsequent differentiation of precursor cells isolated from the neuroepithelium of embryonic day 10 mice in vitro. Here we show that fibroblast growth factor-induced proliferation is dependent on the presence of insulin-like growth factors (IGFs) and that IGF-I is endogenously produced by the neuroepithelial cells. Blocking of endogenous IGF-I activity with anti-IGF-I antibodies results in complete inhibition of fibroblast growth factor-mediated proliferation and in cell death. IGF-I alone acts as a survival agent. These observations correlate with the detection of transcripts for IGF-I and basic fibroblast growth factor in freshly isolated neuroepithelium and are consistent with an autocrine action of these factors in early brain development in vivo.
成纤维细胞生长因子可刺激从胚胎第10天小鼠神经上皮分离的前体细胞在体外增殖并随后分化。我们在此表明，成纤维细胞生长因子诱导的增殖依赖于胰岛素样生长因子（IGF）的存在，并且IGF-I是由神经上皮细胞内源性产生的。用抗IGF-I抗体阻断内源性IGF-I活性会导致成纤维细胞生长因子介导的增殖完全受到抑制并导致细胞死亡。单独的IGF-I可作为一种存活因子。这些观察结果与在新鲜分离的神经上皮中检测到IGF-I和碱性成纤维细胞生长因子的转录本相关，并且与这些因子在体内早期脑发育中的自分泌作用一致。
