Risau W
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3855-9. doi: 10.1073/pnas.83.11.3855.
Embryonic chicken brain was analyzed for the presence of growth factors for capillary endothelial cells. A growth factor was found whose total and specific activities began to increase around the 12th day of development and reached a plateau around the 14th-16th day. A high specific activity was observed early in embryonic development at days 3 and 4. The growth factor was partially purified from extracts of 18-day-old embryonic chicken brain by heparin-Sepharose affinity chromatography where it eluted as a single peak of activity at 1.2-1.4 M sodium chloride. In chromatography on Sephacryl S200 gels, the activity eluted at a molecular size of 16- to 18-kDa. The growth factor is mitogenic for endothelial cells but not for smooth muscle or glial cells. In vivo assays using rabbit cornea and chicken chorioallantoic membrane demonstrated the angiogenic capacity of the chromatographed growth factor. The relationship between this early factor, the subsequent invasion of blood vessels into neural tissue, and the formation of the blood-brain barrier is discussed.
对鸡胚脑进行分析，以检测其中是否存在针对毛细血管内皮细胞的生长因子。发现了一种生长因子，其总活性和比活性在发育第12天左右开始增加，并在第14 - 16天左右达到平台期。在胚胎发育早期的第3天和第4天观察到高比活性。通过肝素 - 琼脂糖亲和层析从18日龄鸡胚脑提取物中对该生长因子进行了部分纯化，在1.2 - 1.4M氯化钠浓度下以单一活性峰洗脱。在Sephacryl S200凝胶上进行层析时，活性在分子大小为16 - 18kDa时洗脱。该生长因子对内皮细胞有促有丝分裂作用，但对平滑肌细胞或神经胶质细胞无此作用。使用兔角膜和鸡绒毛尿囊膜进行的体内试验证明了经层析的生长因子具有血管生成能力。本文讨论了这种早期因子、随后血管向神经组织的侵入以及血脑屏障形成之间的关系。
